Legacy Technologies 

Sanger Sequencing and Fragment Analysis

As announced in December 2019, the University of Michigan Advanced Genomics Core discontinued all Sanger sequencing services on March 13, 2020. The U of M research community will be better served by externals vendors for commodity Sanger needs, and the change will allow the AGC to focus on maximizing the added value of high throughput sequencing and related genomic techniques.

Sanger sequencing services are available to Michigan researchers through UM-wide relationships with Eurofins Genomics and GeneWiz.

Genewiz

Procurement Services announced that effective Monday, February 1, 2021 that Genewiz is now a contract supplier on the University’s M-marketsite ordering platform.  Their punch-out catalog enables you to quickly and conveniently place your Sanger Sequencing orders via M-marketsite. Instructions detailing how to place orders, contact information, and dropbox locations can be found on the Genewiz Procurement Page.

Eurofins

Procurement Services announced that effective Monday, June 1, 2020 that Eurofins is now a contract supplier on the University’s M-marketsite ordering platform.  Their punch-out catalog enables you to quickly and conveniently place your Sanger Sequencing orders via M-marketsite. Instructions detailing how to place orders, contact information, and dropbox locations can be found on Eurofins Procurement Page.

Legacy FAQs

 

Affymetrix

The University of Michigan Advanced Genomics Core discontinued Affymetrix services July 2019. A list of certified Affymetrix service providers is available here: https://www.thermofisher.com/us/en/home/life-science/microarray-analysis/microarray-analysis-partners-programs/affymetrix-service-providers.html.  

Alternative Technologies

QuantSeq provides cost-efficient, gene expression profiling data without the need for polyA-enrichment or rRNA depletion. Next-generation sequencing-based, reads are generated towards the poly(A) tail and directly correspond to the mRNA sequence simplifying the bioinformatic analysis. For gene expression studies, QuantSeq is an excellent alternative to microarrays and standard RNA-seq. 

 

 

Sequenom

The University of Michigan Advanced Genomics Core discontinued all Sequenom genotyping services Spring 2019. Sequenom services are available from Agena Bioscience (formerly Sequenom) https://agenabio.com/services/assays-by-agena/.

Alternative Technologies

Design Taqman assays. You would need to set up the plates, but the core does have the QuantStudio needed so we can assist with running the PCR.

Custom NGS panel. You would work with a company to design a panel of amplicons that cover your SNPs of interest. The AGC can perform both the prep and accommodate the sequencing. Contact us for vendor recommendations

 

PacBio

The University of Michigan Advanced Genomics Core discontinued PacBio services Summer 2018. A list of certified PacBio service providers is available here: https://www.pacb.com/products-and-services/service-providers/.  

Alternative Technologies

Oxford Nanopore Technology – The Advanced Genomics Core houses a GridION instrument. Please contact us for more information.

Linked-Read library prep barcodes HMW DNA prior to building an Illumina compatible DNA library. Analysis pipelines deconvolute the HMW barcodes to stitch the short reads from the sequencer together to generate synthetic long reads and enables phased and/or structural variant detection. The AGC can accommodate the entire workflow – prep, sequencing, and data processing. Contact us for more information.

 

Translate »