Real-Time PCR
close up of Real-time PCR equipment

Information about qPCR instrument rental and PCR array.

QPCR Instrument Rental

The Advanced Genomics Core allows reservations on our QuantStudio Real-Time PCR system. To run real-time PCR plates at the AGC, initiate a new Quantitative Real-time PCR request in MiCores. The ready-to-run plates need to be dropped off at NCRC or placed in the MSRB II real-time plate drop-off refrigerator by 2pm.

Required Consumables

  • MicroAmp Optical 384-well Reaction Plate (cat# 4343370)
  • MicroAmp Optical Adhesive Film (cat# 4311971)

The plates must be compatible with the instrument and be in ready-to-run condition when you drop them off.

Default Run Settings

scientific chart of QuantStudio Real-Time PCR, Default Run Settings

 

Your results will be uploaded to your MiCores request within 24-72 hours from plate submission. The data (Ct-values) will be in the form of an excel spreadsheet (.xlsx file) as well as in instrument specific format (.eds) that will require the instrument’s software to open. Please note that the Ct values will be in the ‘Results’ sheet at the bottom of the excel file.

Questions?
Contact Us
Room 122
NCRC Building 14
University of Michigan
2800 Plymouth Rd.
Ann Arbor, MI 48109-2800
About Us
The Advanced Genomics Core is one of the Biomedical Research Core Facilities, and a part of the Medical School Office of Research, where our mission is to foster an environment of innovation and efficiency that serves the Michigan Medicine research community and supports biomedical science from insight to impact.