Mission Bio Single-Cell Processing
The Mission Bio Tapestri instrument uses a two-step microfluidic workflow to access DNA and proteins in single cells. DNA and oligo-conjugates are first isolated from the cells. Next, multiplex PCR is used to amplify targets.
Image provided by Mission Bio
Sample Requirements
| Workflow | DNA Only | DNA + Protein |
| Cell Viability | >80% | >90% |
| Cell concentration | 3000-4000 cells/μl | 6000-10000 cells/μl |
| Total volume | 50 μl | 100 μl |
Book an appointment! Detailed sample submission instructions can be downloaded here. Single-cell data are analyzed with the Tapestri Pipeline.
**Build-to-order and custom options also available. Information can be found on here.**
DNA panels can be combined with BioLegend TotalSeq-D antibodies to obtain genotype and phenotype data from the same cells across thousands of cells.
Frequently Asked Questions
Can I have my cells checked without running my full experiment?
Yes, we offer viability only appointment slots for you to check your cell dissociation method before using experimental cells. Select “viability/count only” as your assay type when filling out the MiCores Single Cell Appointment request form. We ask that you bring a maximum of four samples for the appointment.
Can I FACS sort my cells before I bring them?
Absolutely. Some things to keep in mind when sorting your cells prior to bringing them:
- FACS will overestimate the number of cells on average by 50%. One way to mitigate this problem is to stain your cells with Ruby. This will separate the cells from debris and make the count much more accurate.
- Your cells may not like to stay in FACS buffer. You can sort into a full media that is compatible with your cells, and resuspend them in a compatible media afterward.
- Your cells will likely be very dilute when they come out of the sorter. You will likely want to concentrate them before coming to the Core.
Can I combine single cell DNA panels with CITE-seq?
Yes! The Tapestri platform is compatible with BioLegend TotalSeq-D antibodies.
Can I fix my cells?
Alcohol-based fixatives, such as acetic acid, EtOH, and MeOH, are compatible with the technology.