Using the Illumina Infinium Methylation EPIC kit, the DNA Sequencing Core performs high throughput sample Methylation profiling for intact and FFPE bisulfite converted DNA samples. The Core Services for the Illumina BeadArray platform consist of the following: 1). perform InfiniumHD Methylation assay, 2). Record signal intensities with an iScan Scanner, 3). create a GenomeStudio Project for data analysis, and 4). generate reports (Internal controls, SamplesTable, SampleMethylationProfile, GroupMethylationProfile).
|Primary Contact||Susan Dagenais, Ph.D.
UM DNA Sequencing Core
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||Methylation profiling of >850,000 CpG markers. For generating Methylation Profiles, the iScan is set to record data that can be used with the Illumina GenomeStudio data analysis software as well as other software such packages such as Bioconductor.|
|How to Request||Sample Submission – Contact Susan Dagenais (firstname.lastname@example.org) and request a Project Quote. Please indicate the number of samples to be assayed and which Illumina BeadChip kit you would like used for the assay. We will e-mail you a Quote for the Illumina Kit costs and estimated Core Processing fees. Once we a signed Quote, we will place an order with Ilumina, assign a Core Project Number, and you will be sent a BeadArray submission form for sample submission.|
|Sample Requirements||The client will submit bi-sulfite converted DNA (8-10ul at 50ng/ul). Bisulfite-converted DNA should be created using the Zymo EZ_DNA_Methylation kit (ZymoResearch) using Illumina incubation conditions (Appendix 6). For FFPE samples, we perform the Illumina FFPE Restoration protocol on bi-sulfite converted DNA submitted by the client.|
|Typical Turnaround||Typical turnaround time is 1-3 weeks.|
|More Information||Illumina Infinium Methylation EPIC Kit
ZymoResearch EZ DNA Methylation Kit