PyroMark CpG Analysis

PyroMark provides real-time sequence information, highly suitable for methylation analysis. PyroMark CpG Assays are the only genomewide, predesigned methylation assays for Pyrosequencing analysis that use a tailored design algorithm for highly specific assay design and successful CpG methylation results. The assay database includes over 84,000 individual assays for gene-specific human, mouse, and rat CpG sites. PyroMark also offers predesigned assays for validating results from methylation arrays such as the HumanMethylation450K BeadChip array.

Platform	PyroMark Q96 MD and PyroMark Q96 Vacuum Workstation
Category	Genotyping
Primary Contact	Ellen Pedersen Research Specialist: Sequenom and PyroMark Services elleped@umich.edu University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134 734-615-5646
Typical Cost	Click here to view internal pricing list! Click here to view external pricing list!
Typical Output	Plate Report (text or Excel file) containing allele quantification percentages and sample/well quality metrics, along with Pyrograms depicting spectra for each well/sample.
How to Request	Contact Dr. Lyons for initial consultation; if he deems project appropriate for PyroMark, contact Ellen Pedersen.
Sample Requirements	Sample prep according to Qiagen’s specifications; Ellen will arrange direct support from Qiagen to ensure optimization of PCR products prior to sample submission for pyrosequencing.
Typical Turnaround	Depends on project queue; each plate/run requires ~4 hours to process.
More Information	If Assay Design is completed by Core, the costs will involve additional labor charges billed at the current approved PyroMark Tech time hourly rate, which will be added to the current per plate recharge rate.