Fragment Size Analysis
Our Fragment Analysis service provides clients the ability to size their DNA fragments using a fluorescence-based detection system on our 3730XL Genetic Analyzer. A maximum of five fluorescent dyes per lane permits multiplexing of four microsatellites and the fifth dye for an internal size standard to correct for variability between lanes.
|Primary Contact||Jodi Atkins
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||Raw data .fsa files are generated from the 96 well CE plate.
GeneMarker software from SoftGenetics is available for clients to check out to analyze fragment analysis data.
|How to Request||Sample Submission: go to https://client-seqcore.brcf.med.umich.edu then choose ‘Submit Fragment Analysis Service, Submit CE plate’|
|Sample Requirements||CE Loading plates submitted by clients must contain a size standard in 12ul/well of Hi- Di Formamide plus 1 ul of sample diluted in water. Clients may choose the number of wells to fill with samples. All wells must have 12ul of a liquid in them (Hi-Di Formamide or water with no sample).|
|Typical Turnaround||Between 1 to 3 days, depending on how much business Sanger sequencing is processing.|
|Critical Information||Contact the core to consult the compatibility of the fluorescent dyes used with your samples and calibration dyes for our 3730XL Genetic Analyzer Instrument. A proper dilution of PCR products in CE Loading plates is necessary to prevent overloading of array capillaries with high-fluorescent signal intensities and could contaminate the next plate run. It is important to Label your CE loading plates with the identification plate number assigned by our database on submission.|
|More Information||Clients must have an account set up with the DNA Sequencing Core prior to submission of samples. The clients can use any of our drop off locations listed on our web page: Sample Drop-Off Locations
CE Plates received from outside the University of Michigan can be shipped to: University of Michigan, DNA Sequencing Core, NCRC Bld 14, Room 122, 2800 Plymouth Road, Ann Arbor, MI 48109-2800. Plates must be shipped on gel ice packs.
CE loading plates prepared and submitted by clients are required to use ABI Optical plates or Optical ABI compatible 96 well plates with a barcode.
Acceptable barcoded plates are: