PyroMark SNP/Allele Quantification Analysis


PyroMark provides real-time sequence information, highly suitable for genetic analysis. It can be used to determine sequence variation–including single nucleotide polymorphisms and insertions/deletions–generating quantifiable results.

PlatformPyroMark SNP/Allele Quantification Analysis
Primary ContactEllen Pedersen
Research Specialist: Sequenom and PyroMark Services
University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134
Typical OutputPlate Report (text or Excel file) containing allele quantification percentages and sample/well quality metrics, along with Pyrograms depicting spectra for each well/sample.
How to RequestContact Dr. Lyons for initial consultation; if he deems project appropriate for PyroMark, contact Ellen Pedersen.
Sample RequirementsSample prep according to Qiagen’s specifications; Ellen will arrange direct support from Qiagen to ensure optimization of PCR products prior to sample submission for pyrosequencing.
Typical TurnaroundDepends on project queue; each plate/run requires ~4 hours to process.
Critical InformationIf Assay Design is completed by Core, the costs will involve additional labor charges billed @ $76/hr, which will be added to the per plate recharge rate of $320.