PyroMark SNP/Allele Quantification Analysis
PyroMark provides real-time sequence information, highly suitable for genetic analysis. It can be used to determine sequence variation–including single nucleotide polymorphisms and insertions/deletions–generating quantifiable results.
| Platform | PyroMark SNP/Allele Quantification Analysis |
| Category | Genotyping |
| Primary Contact | Ellen Pedersen Research Specialist: Sequenom and PyroMark Services elleped@umich.edu University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134 734-615-5646 |
| Typical Output | Plate Report (text or Excel file) containing allele quantification percentages and sample/well quality metrics, along with Pyrograms depicting spectra for each well/sample. |
| How to Request | Contact Dr. Lyons for initial consultation; if he deems project appropriate for PyroMark, contact Ellen Pedersen. |
| Sample Requirements | Sample prep according to Qiagen’s specifications; Ellen will arrange direct support from Qiagen to ensure optimization of PCR products prior to sample submission for pyrosequencing. |
| Typical Turnaround | Depends on project queue; each plate/run requires ~4 hours to process. |
| Critical Information | If Assay Design is completed by Core, the costs will involve additional labor charges billed @ $76/hr, which will be added to the per plate recharge rate of $320. |