RT2 Profiler Targeted Gene Expression Analysis
SABiosciences, A QIAGEN Company, offers RT² Profiler PCR Arrays. These targeted arrays analyze expression of a panel of 84 genes associated with any one of over 150 biological pathways or specific disease states. PCR Arrays are the most reliable tools for analyzing the expression of a focused panel of genes. Each 384-well plate PCR Array includes SYBR Green-optimized primer assays for a thoroughly researched panel of relevant, pathway- or disease-focused genes. PCR Arrays can also be customized to contain a panel of genes tailored to your specific research interests.
|Platform||Qiagen RT2 Profiler Array Plates|
|Category||Gene Expression Analysis|
|Primary Contact||Christopher Krebs, Manager, UM DNA Sequencing Core
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||Report containing statistical assessment of comparative groups is provided along with the raw Ct data in format compatible with Microsoft Excel.|
|How to Request||Login through https://client-seqcore.brcf.med.umich.edu and then choose “Sample QC” to submit samples. On the page showing Service Requested, select one of the Bioanalyzer RNA chip options. In the “Reason” pull-down menu, choose “Targeted PCR based array submission”. Add additional information (e.g., “Please run Human Breast Cancer Array PAHS-131Z”, “Samples are for Mouse Angiogenesis”, etc.) in the “Additional Notes” section.|
|Sample Requirements||A minimum of 100 ng total RNA for Standard Processing
A minimum of 25 ng total RNA for High Sensitivity Processing
|Typical Turnaround||3 to 4 weeks, however, timing is dependent on availability of arrays, which are purchased as needed.|
|Critical Information||RNA quality and concentration are confirmed for an additional fee (see QC FAQ) before beginning any Microarray project. Please consult with the Manager before submitting samples that are known or suspected to be of low concentration or highly degraded (i.e., from FFPE sources).|
|More Information||For a list of arrays available, product descriptions and additional technical information, go to the Qiagen website: https://www.qiagen.com/us/shop/pcr/primer-sets/rt2-profiler-pcr-arrays/
For more information about RNA quality checks, go to: FAQ for QC
For other options for gene expression analysis (e.g., RNA-seq) go to: FAQ for Next Generation Sequencing (NGS).