Human Cell Line Validation
The Fragment Analysis service offers the AMPFLSTR Identifiler Plus Assay for cell line identification sold by Applied Biosystems. This assay does identify Human Genomic DNA for 15 tetranucleotide repeat loci and the Amelogenin gender determination marker. Our 3730XL Genetic Analyzer uses five fluorescent dyes per lane permitting a 16 locus multiplex and a fifth dye for an internal size standard to correct for variability between lanes.
|Category||Microsatellite Genotyping-Human Cell Line Validation-CODIS|
|Primary Contact||Jodi Atkins
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||Raw data .fsa files are generated for each sample. GeneMapper software is used to analyze the data and .pdf documents are generated of the analyzed data. HID GeneMarker software from SoftGenetics is available for clients to check out and analyze data themselves.|
|How to Request||Sample Submission: go to https://client-seqcore.brcf.med.umich.edu then choose ‘Fragment Analysis Service, Submit Samples for IdentifilerPlus’.|
|Sample Requirements||Submit 20ul of DNA sample at a concentration of 0.1 ng/ul in Ultra pure RNase, DNase free water or EB Buffer.|
|Typical Turnaround||Between 1 to 3 days. The Identifiler Plus samples take a couple of days to process.|
|Critical Information||Submit samples in 1.5 ml microcentrifuge tubes. It is important to Label each tubes with the identification number assigned by our database on submission of your samples.|
|More Information||The HID GeneMarker software is available to check out at room C562 MSRB II.
Clients must have an account set up with the DNA Sequencing Core prior to submission of samples. The clients can use any of our drop off locations listed on our web page: http://seqcore.brcf.med.umich.edu/.
Identifiler Plus samples received from outside the University of Michigan can be shipped to: University of Michigan, DNA Sequencing Core, NCRC Bld 14, Room 122, 2800 Plymouth Road, Ann Arbor, MI 48109-2800.