Sequenom iPLEX SNP Genotyping
The Sequenom® platform utilizes MALDI-TOF mass spectrometry in a cost-effective method that assays tens to hundreds of SNPs/indels in hundreds to thousands of samples, with multi-plexing capabilities as high as 40 targets per reaction.
|Platform||MassARRAY® (Sequenom, dba Agena Bioscience)|
|Primary Contact||Ellen Pedersen
Research Specialist: Sequenom and PyroMark Services
University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||Excel files containing allele calls—separate file for each assay, delivered via email.|
|How to Request||Contact Dr. Lyons for initial consultation; if he deems project appropriate for Sequenom, contact Ellen Pedersen.|
|Sample Requirements||For each SNP-plex reaction, we need 5-10uL of 50-100ng/uL genomic DNA if quantitated on nanodrop, 25-50ng/uL if quantitated with picogreen on fluorimeter.|
|Typical Turnaround||Depends on number of samples and number of targets to be assayed.|
|Critical Information||The fixed costs described above do not include labor, which is charged at the current approved rate and estimated as follows:
|More Information||All custom primer orders are billed to a short code provided by the client: e.g., the interrogation of 40 variants requires 3 primers per target = 120 primers @ ~$5 per primer = ~$600. This order would yield enough primer to investigate at least 1,000 samples.|