Sequenom iPLEX SNP Genotyping

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The Sequenom® platform utilizes MALDI-TOF mass spectrometry in a cost-effective method that assays tens to hundreds of SNPs/indels in hundreds to thousands of samples, with multi-plexing capabilities as high as 40 targets per reaction.

PlatformMassARRAY® (Sequenom, dba Agena Bioscience)
CategoryGenotyping
Primary ContactEllen Pedersen
Research Specialist: Sequenom and PyroMark Services
elleped@umich.edu
University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134
734-615-5646
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical OutputExcel files containing allele calls—separate file for each assay, delivered via email.
How to RequestContact Dr. Lyons for initial consultation; if he deems project appropriate for Sequenom, contact Ellen Pedersen.
Sample RequirementsFor each SNP-plex reaction, we need 5-10uL of 50-100ng/uL genomic DNA if quantitated on nanodrop, 25-50ng/uL if quantitated with picogreen on fluorimeter.
Typical TurnaroundDepends on number of samples and number of targets to be assayed.
Critical InformationThe fixed costs described above do not include labor, which is charged at the current approved rate and estimated as follows:

  • Assay design—2 to 4 hours, depending on number of targets

  • Sample processing—4 to 6 hours, depending on number of samples/SNPs

  • Reaction spotting—2 to 4 hours, depending on total number of chips

  • Data analysis—2 to 4 hours per chip

More InformationAll custom primer orders are billed to a short code provided by the client: e.g., the interrogation of 40 variants requires 3 primers per target = 120 primers @ ~$5 per primer = ~$600. This order would yield enough primer to investigate at least 1,000 samples.