NextGen Bisulfite Sequencing
Detection and quantification of methylation. Methylated DNA is treated with bisulfite which converts unmethylated cytosines to uracil. Methylated cytosines remain unchanged. This service yields the bisulfite-converted genomic libraries, but sequencing is an extra expense. Please consult with your bioinformaticist to determine appropriate parameters for sequencing, and required depth of sequencing.
|Platform||Illumina HiSeq Libraries|
|Typical Cost|| Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||Illumina ready library|
|How to Request||Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’|
|Sample Requirements||1 to 3 ug of good quality DNA in 25-50ul|
|Critical Information||Libraries will have slightly lower diversity.|