NextGen Bisulfite Sequencing

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Detection and quantification of methylation. Methylated DNA is treated with bisulfite which converts unmethylated cytosines to uracil. Methylated cytosines remain unchanged. This service yields the bisulfite-converted genomic libraries, but sequencing is an extra expense. Please consult with your bioinformaticist to determine appropriate parameters for sequencing, and required depth of sequencing.

PlatformIllumina HiSeq Libraries
CategoryRRBS
Primary Contactillumina-manager@umich.edu
Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical OutputIllumina ready library
How to RequestSample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’
Sample Requirements1 to 3 ug of good quality DNA in 25-50ul
Critical InformationLibraries will have slightly lower diversity.