Rapid Paired end 125bp Sequencing


Rapid mode paired-end sequencing on the HiSeq 2500 that produces paired 125 bp reads.

PlatformIllumina HiSeq Sequencing
CategoryRapid Paired-end 125bp Sequencing
Primary Contactillumina-manager@umich.edu
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical Output45 Gb per lane
How to RequestSample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’
Sample Requirements30 ul of 10nM Illumina library with an insert size of at least 350 bp
Typical TurnaroundThe run itself takes 32 hours the flow cell needs to be full for us to run it. Each flow cell has two lanes and we need to have a rapid mode HiSeq available. This could take some time to fill and run.
Critical InformationRapid modes come at a premium while they are much faster run times they produce about 80% of the data as compared to a high output run.