Rapid Paired end 150bp Sequencing
Rapid mode paired-end sequencing on the HiSeq 2500 that produces paired 150 bp reads.
|Platform||Illumina HiSeq Sequencing|
|Category||Rapid Paired-end 150bp Sequencing|
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||40 Gb per lane|
|How to Request||Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’|
|Sample Requirements||30 ul of 10nM Illumina library with an insert size of at least 350 bp|
|Typical Turnaround||The run itself takes 40 hours the flow cell needs to be full for us to run it. Each flow cell has two lanes and we need to have a rapid mode HiSeq available. This could take some time to fill and run.|
|Critical Information||Rapid modes come at a premium while they are much faster run times they produce about 80% of the data as compared to a high output run.|