Small RNA Seq library prep
Preparation of total RNA for small RNA Sequencing. Profiling micro RNAs and other non-coding RNA for any organism. Total RNA or purified small RNA fragments are adapter ligated and reverse transcribed, PCR amplified and gel purified.
|Platform||Illumina HiSeq 2500, NextSeq, and MiSeq.
|Category||Small RNA Sequencing|
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||Illumina ready library.|
|How to Request||Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’|
|Sample Requirements||Good quality total RNA 100ng in 10ul|
|Critical Information||Only profiling of small RNA can be done using this prep.|
|Additional Information||Please discuss your RNA Seq project with your bioinformaticist or contact the University of Michigan's Bioinformatics Core (email@example.com) before you begin your project.
For kit information please see NEB's site:
Due to limited space in the cores freezer's we can only keep samples for 6 months. If you would like your samples returned to you after sequencing please contact the core.