Small RNA Seq library prep

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Preparation of total RNA for small RNA Sequencing. Profiling micro RNAs and other non-coding RNA for any organism. Total RNA or purified small RNA fragments are adapter ligated and reverse transcribed, PCR amplified and gel purified.

PlatformIllumina HiSeq 2500, NextSeq, and MiSeq.
CategorySmall RNA Sequencing
Primary Contactillumina-manager@umich.edu
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical OutputIllumina ready library.
How to RequestSample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’
Sample RequirementsGood quality total RNA 100ng in 10ul
Critical InformationOnly profiling of small RNA can be done using this prep.
Additional InformationPlease discuss your RNA Seq project with your bioinformaticist or contact the University of Michigan's Bioinformatics Core (bioinformatics@umich.edu) before you begin your project.

For kit information please see NEB's site:
https://www.neb.com/products/e7330-nebnext-small-rna-library-prep-set-for-illumina-multiplex-compatible#Product%20Information

Due to limited space in the cores freezer's we can only keep samples for 6 months. If you would like your samples returned to you after sequencing please contact the core.