Ultra low non stranded mRNA Seq SMARTer kit

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Preparation of RNA samples for sequencing on the Illumina HiSeq. Described here is a library preparation service only. Once the library is prepared, you will also need to request (and pay for) the sequencing of that library.

Total RNA is first amplified using the SMARTer technology and made into cDNA.

The cDNA is input into the Rubicon Thruplex library prep where it is end repaired A tailed and Illumina adapters are ligated.

The resultant library is then PCR amplified to have enough material for the sequencer.

PlatformIllumina HiSeq 2500, HiSeq 4000, NextSeq, and MiSeq.
CategoryGene Expression Profiling
Primary Contactillumina-manager@umich.edu
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical OutputThe SMARTer non-stranded kit will yield 2-10ng of cDNA between 400 and 100 bp 20-50ul of prepared library
How to RequestSample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’
Please deliver RNA to NCRC. Courier service from other sites will not guarantee proper temp is maintained!
Sample Requirements10pg – 10ng total RNA
Critical InformationThe SMARTer protocol has been optimized for cDNA synthesis starting from 10pg of total RNA. However if you RNA sample is not limiting, Clontech recommends that you start with more total RNA (up to 10 ng). Purified total RNA should be in nuclease free water.
Additional InformationDue to the two rounds of amplification in this prep the duplicate rate will be higher than normal RNA Seq libraries.

Please discuss your RNA Seq project with your bioinformaticist or contact the University of Michigan's Bioinformatics Core (bioinformatics@umich.edu) before you begin your project.

For kit information please see Clontech's site:

http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/Next_Gen_Sequencing_Kits/Single_cell_RNA_Seq_Kits_for_mRNA_seq/Single_Cell_RNA_Seq_v4

Due to limited space in the cores freezer's we can only keep samples for 3 months. If you would like your samples returned to you after sequencing please contact the core.