Single end 50 cycle sequencing


Single end sequencing on the HiSeq 2500 and HiSeq 4000 that produces 50 bp reads.

*HiSeq 2500 is being discontinued at the end of 2018, the core will continue to run it until it is no longer feasible.

PlatformIllumina HiSeq 2500 and HiSeq 4000.
CategorySingle end 50 cycle sequencing
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical Output10 Gb per lane from the HiSeq 2500, and 13 Gb per lane from the HiSeq 4000

*these are just estimates of the output, these are not guarantees.
How to RequestSample Submission: login through then choose ‘Illumina Sequencing’
Sample Requirements30 ul of 10nM Illumina library with an insert size of at least 50 bp.
Run TimeThe run itself takes 2.5 days on the HiSeq 2500 and 1 day on the HiSeq 4000.
Critical InformationIt is important that we know if you are working with an unbalanced genome or have modified your library prep in any way.

Please include this information in the notes section when creating a submission.