Ultra low input gDNA
Ultra low genomic DNA (Thruplex Rubicon/ Low input Library prep Clontech)
Preparation of DNA for NGS Described here is a library preparation service only. Once the library is prepared, you will also need to request (and pay for) the sequencing of that library.
DNA is fragmented, end repaired, A-tailed and the library is amplified by PCR with the Illumina Indexing Primer set.
|Platform||Illumina HiSeq 2500, HiSeq 4000, NextSeq, and MiSeq.
|Category||Genome Sequencing (and gene expression profiling if used in conjunction with SMARTer kit)|
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||The Rubicon ThruPlex/Clontech Low input library prep kit will yield enough library to sequence on an Illumina HiSeq|
|How to Request||Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’|
|Sample Requirements||The total fraction of 50pg – 50ng full length, of fragmented double strand DNA.|
|Critical Information||DNA is sheared by sonication on the Covaris. Sheared DNA is end repaired adapter ligated and PCR amplified.|