Ultra low input gDNA

Ultra low genomic DNA (Thruplex Rubicon/ Low input Library prep Clontech)

Preparation of DNA for NGS Described here is a library preparation service only. Once the library is prepared, you will also need to request (and pay for) the sequencing of that library.

DNA is fragmented, end repaired, A-tailed and the library is amplified by PCR with the Illumina Indexing Primer set.

PlatformIllumina HiSeq 2500, HiSeq 4000, NextSeq, and MiSeq.
CategoryGenome Sequencing (and gene expression profiling if used in conjunction with SMARTer kit)
Primary Contactillumina-manager@umich.edu
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical OutputThe Rubicon ThruPlex/Clontech Low input library prep kit will yield enough library to sequence on an Illumina HiSeq
How to RequestSample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’
Sample RequirementsThe total fraction of 50pg – 50ng full length, of fragmented double strand DNA.
Critical InformationDNA is sheared by sonication on the Covaris. Sheared DNA is end repaired adapter ligated and PCR amplified.
Additional Information
  1. Due to limited space in the cores freezer's we can only keep samples for 3 months. If you would like your samples returned to you after sequencing please contact the core.

  2. Due to size limitations on the HiSeq 4000, libraries with a average bp great than 580bp are not recommended to run on the HiSeq 4000.