DNA/RNA QC : Agilent Bioanalyzer / TapeStation
The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA and RNA. The platform is the industry standard for RNA sample QC and has mostly replaced labor-intensive gel electrophoresis.
|Platform||Agilent Bioanalyzer and TapeStation|
|Category||DNA and RNA QC|
|Primary Contact||Christopher Krebs, Manager, UM DNA Sequencing Core
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||You will get back a PDF report that contains the samples electropherogram and pseudo-gel images, as well as a concentration and, for RNA, a RIN value.|
|How to Request||Bring your samples to C562 MSRB II or the DNA Sequencing Core in Building 14, NCRC where you will be asked to fill in contact and billing (shortcode) information on an electronic submission form.|
|Sample Requirements||We request a minimum of 5ul of each sample for analysis by Bioanalyzer. The samples for “QC only” should be in a separate tube from the portion of sample intended for other services within the core. This assists us in minimizing freeze thaw cycles.|
|Typical Turnaround||24 to 48 hrs|
|More Information||The RIN, or RNA Integrity Number, is a method for determining RNA quality. RIN is calculated algorithmically by including the 28s/18s ratio, the region before the peaks, signal areas, and intensities. These calculations enable use of the whole graph to make RNA quality assessments. We make an initial assessment of total RNA QC based on 260/280 and 260/230 ratios. However, we place the highest reliance for QC on the Bioanalyzer integrity assessment. Each sample is assigned a RIN score: 10 corresponds to a pure, un-degraded total RNA sample while a RIN of 1 corresponds to a completely degraded sample. The core recommends only using samples with RIN's greater than 7.
Link to additional information for interpreting Agilent 2100 Bioanalyzer data: http://www.urmc.rochester.edu/fgc/documents/Bioanalyzer_Interpretations_forcustomers.pdf