DNA/RNA QC : Agilent Bioanalyzer / TapeStation

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The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA and RNA. The platform is the industry standard for RNA sample QC and has mostly replaced labor-intensive gel electrophoresis.

PlatformAgilent Bioanalyzer and TapeStation
CategoryDNA and RNA QC
Primary ContactChristopher Krebs, Manager, UM DNA Sequencing Core
734-763-8891 ckrebs@umich.edu
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical OutputYou will get back a PDF report that contains the samples electropherogram and pseudo-gel images, as well as a concentration and, for RNA, a RIN value.
How to RequestBring your samples to C562 MSRB II or the DNA Sequencing Core in Building 14, NCRC where you will be asked to fill in contact and billing (shortcode) information on an electronic submission form.
Sample RequirementsWe request a minimum of 5ul of each sample for analysis by Bioanalyzer. The samples for “QC only” should be in a separate tube from the portion of sample intended for other services within the core. This assists us in minimizing freeze thaw cycles.
Typical Turnaround24 to 48 hrs
Critical InformationBioanalyzer

  • RNA 6000 Nano Kit: 5-500 ng/ul, RNA Size range: 25-6000 nt

  • RNA 6000 Pico Kit: 50-5000 pg/ul RNA Size range: 25-6000 nt

  • RNA small RNA Kit: 1-100 ng/ul RNA Size range: 6-150 nt

  • DNA 1000 Kit: 0.1-50 ng/ul DNA Size range: 25-1000 bp

  • DNA 12K Kit: 0.5-50 ng/ul DNA Size range: 100-12000 bp

  • DNA High Sensitivity Kit: 5-500 pg/ul DNA Size range: 50-7000 bp


TapeStation

  • RNA ScreenTape: 25-500 ng/ul, RNA Size range: 100-6000 nt

  • High Sensitivity RNA ScreenTape: 1-25 ng/ul RNA Size range: 100-6000 nt

  • DNA 1K ScreenTape: 0.1-50 ng/ul DNA Size range: 35-1000 bp

  • High Sensitivity DNA ScreenTape: 10-1000 pg/ul DNA Size range: 35-1000 bp

  • Genomic DNA ScreenTape: 5-300 ng/ul DNA Size range: 200-60,000 bp

More InformationThe RIN, or RNA Integrity Number, is a method for determining RNA quality. RIN is calculated algorithmically by including the 28s/18s ratio, the region before the peaks, signal areas, and intensities. These calculations enable use of the whole graph to make RNA quality assessments. We make an initial assessment of total RNA QC based on 260/280 and 260/230 ratios. However, we place the highest reliance for QC on the Bioanalyzer integrity assessment. Each sample is assigned a RIN score: 10 corresponds to a pure, un-degraded total RNA sample while a RIN of 1 corresponds to a completely degraded sample. The core recommends only using samples with RIN's greater than 7.

Link to additional information for interpreting Agilent 2100 Bioanalyzer data: http://www.urmc.rochester.edu/fgc/documents/Bioanalyzer_Interpretations_forcustomers.pdf