DNA/RNA QC : Nanodrop Spec
The NanoDrop UV-Vis Spectrophotometer is capable of measuring the concentration of nucleic acids using a full spectrum of absorption data.
|Category||DNA and RNA QC|
|Primary Contact||Christopher Krebs, Manager, UM DNA Sequencing Core
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||You will get back an Excel spreadsheet report that contains the sample concentration and photometric values across several wavelengths, including O.D. 260/280 and O.D. 260/230 ratios to assess purity.|
|How to Request||Bring your samples to C562 MSRB II or the DNA Sequencing Core in Building 14, NCRC where you will be asked to fill in contact and billing (shortcode) information on an electronic submission form.|
|Sample Requirements||We request a minimum of 5ul of each sample for analysis by NanoDrop. The samples for “QC only” should be in a separate tube from the portion of sample intended for other services within the core. This assists us in minimizing freeze thaw cycles.|
|Typical Turnaround||24 to 48 hrs|
5 - 3700 ng/ ul dsDNA
5 - 3000 ng/ ul RNA
5 - 2400 ng/ ul ssDNA
|More Information||O.D.260/280: a ratio of ~1.8 is accepted as “pure” DNA and a ratio of ~2.0 is accepted as “pure” RNA. Phenol or protein contamination will appreciably lower this ratio.
O.D.260/230: a ratio above the O.D.260/280 ratio is accepted as “pure” nucleic acid. Typical values vary from 1.8 to 2.2. Appreciably lower values may indicate the presence of co-purified contaminants.
Link to additional information about NanoDrop: http://www.nanodrop.com/Productnd2000overview.aspx