DNA/RNA QC : Nanodrop Spec
The NanoDrop UV-Vis Spectrophotometer is capable of measuring the concentration of nucleic acids using a full spectrum of absorption data.
|Category||DNA and RNA QC|
|Primary Contact||Christopher Krebs, Manager, UM DNA Sequencing Core
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||You will get back an Excel spreadsheet report that contains the sample concentration and photometric values across several wavelengths, including O.D. 260/280 and O.D. 260/230 ratios to assess purity.|
|How to Request||Login through https://client-seqcore.brcf.med.umich.edu and then choose “Sample QC” to submit samples|
|Sample Requirements||We request a minimum of 5ul of each sample for analysis by NanoDrop. The samples for “QC only” should be in a separate tube from the portion of sample intended for other services within the core. This assists us in minimizing freeze thaw cycles.|
|Typical Turnaround||24 to 48 hrs|
5 - 3700 ng/ ul dsDNA
5 - 3000 ng/ ul RNA
5 - 2400 ng/ ul ssDNA
|More Information||O.D.260/280: a ratio of ~1.8 is accepted as “pure” DNA and a ratio of ~2.0 is accepted as “pure” RNA. Phenol or protein contamination will appreciably lower this ratio.
O.D.260/230: a ratio above the O.D.260/280 ratio is accepted as “pure” nucleic acid. Typical values vary from 1.8 to 2.2. Appreciably lower values may indicate the presence of co-purified contaminants.
Link to additional information about NanoDrop: http://www.nanodrop.com/Productnd2000overview.aspx