DNA/RNA QC : Nanodrop Spec


The NanoDrop UV-Vis Spectrophotometer is capable of measuring the concentration of nucleic acids using a full spectrum of absorption data.

PlatformNanoDrop Spectrophotometer
CategoryDNA and RNA QC
Primary ContactChristopher Krebs, Manager, UM DNA Sequencing Core
734-763-8891 ckrebs@umich.edu
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical OutputYou will get back an Excel spreadsheet report that contains the sample concentration and photometric values across several wavelengths, including O.D. 260/280 and O.D. 260/230 ratios to assess purity.
How to RequestLogin through https://client-seqcore.brcf.med.umich.edu and then choose “Sample QC” to submit samples
Sample RequirementsWe request a minimum of 5ul of each sample for analysis by NanoDrop. The samples for “QC only” should be in a separate tube from the portion of sample intended for other services within the core. This assists us in minimizing freeze thaw cycles.
Typical Turnaround24 to 48 hrs
Critical InformationSensitivity:
5 - 3700 ng/ ul dsDNA
5 - 3000 ng/ ul RNA
5 - 2400 ng/ ul ssDNA
More InformationO.D.260/280: a ratio of ~1.8 is accepted as “pure” DNA and a ratio of ~2.0 is accepted as “pure” RNA. Phenol or protein contamination will appreciably lower this ratio.
O.D.260/230: a ratio above the O.D.260/280 ratio is accepted as “pure” nucleic acid. Typical values vary from 1.8 to 2.2. Appreciably lower values may indicate the presence of co-purified contaminants.
Link to additional information about NanoDrop: http://www.nanodrop.com/Productnd2000overview.aspx