DNA/RNA QC : Qubit Fluorometer
The Qubit 2.0 Fluorometer is capable of measuring the concentration of nucleic acids using Life Technologies Qubit® DNA and RNA quantitation assays. In contrast to the nanodrop, The Qubit® Quantitation Platform, uses fluorescent dyes to measure the concentration of the specific molecules of interest (dsDNA, total nucleic acid, RNA).
| Platform | Qubit 2.0 Fluorometer |
| Category | DNA and RNA QC |
| Primary Contact | Christopher Krebs, Manager, UM DNA Sequencing Core 734-763-8891 ckrebs@umich.edu |
| Typical Cost | Click here to view internal pricing list! Click here to view external pricing list! |
| Typical Output | You will get back the sample's concentration in a Microsoft Excel spreadsheet. |
| How to Request | Login through https://client-seqcore.brcf.med.umich.edu and then choose “Sample QC” to submit samples |
| Sample Requirements | We request a minimum of 5ul of each sample for analysis. The samples for “QC only” should be in a separate tube from the portion of sample intended for other services within the core. This assists us in minimizing freeze thaw cycles. |
| Typical Turnaround | 24 to 48 hrs |
| Critical Information | Assay Ranges: Qubit RNA Assay: 0.25 - 100 ng/ul Qubit High Sensitivity dsDNA Assay: 0.01 - 100 ng/ul Qubit Broad Range dsDNA Assay: 0.1 – 1000 ng/ul Qubit ssDNA Assay: 0.05 – 200 ng/ul |
| More Information | The Qubit® 2.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. Qubit® fluorometric quantitation provides the most specific and sensitive DNA and RNA quantitation available, even at low concentrations Link to additional information about the Qubit : Qubit® 2.0 Fluorometer |