DNA/RNA QC : Qubit Fluorometer
The Qubit 2.0 Fluorometer is capable of measuring the concentration of nucleic acids using Life Technologies Qubit® DNA and RNA quantitation assays. In contrast to the nanodrop, The Qubit® Quantitation Platform, uses fluorescent dyes to measure the concentration of the specific molecules of interest (dsDNA, total nucleic acid, RNA).
|Platform||Qubit 2.0 Fluorometer|
|Category||DNA and RNA QC|
|Primary Contact||Christopher Krebs, Manager, UM DNA Sequencing Core
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||You will get back the sample's concentration in a Microsoft Excel spreadsheet.|
|How to Request||Login through https://client-seqcore.brcf.med.umich.edu and then choose “Sample QC” to submit samples|
|Sample Requirements||We request a minimum of 5ul of each sample for analysis. The samples for “QC only” should be in a separate tube from the portion of sample intended for other services within the core. This assists us in minimizing freeze thaw cycles.|
|Typical Turnaround||24 to 48 hrs|
|Critical Information||Assay Ranges:
Qubit RNA Assay: 0.25 - 100 ng/ul
Qubit High Sensitivity dsDNA Assay: 0.01 - 100 ng/ul
Qubit Broad Range dsDNA Assay: 0.1 – 1000 ng/ul
Qubit ssDNA Assay: 0.05 – 200 ng/ul
|More Information||The Qubit® 2.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. Qubit® fluorometric quantitation provides the most specific and sensitive DNA and RNA quantitation available, even at low concentrations
Link to additional information about the Qubit : Qubit® 2.0 Fluorometer