Multiplexing

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PlatformIllumina Sequencing Libraries on the HiSeq 2500, HiSeq 4000, MiSeq, NextSeq, and NovaSeq
CategoryMultiplexing
Primary Contactillumina-manager@umich.edu
Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
How to RequestSample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’​​
Sample RequirementsMore than one library per sequencing lane.
Critical InformationTo achieve a similar number of reads per sample special care must be taken when pooling samples for sequencing. Samples are carefully quantified, balanced and pooled.

Barcode balance is another important aspect of multiplexing. Illumina has guide lines for each of there different barcodes sets on the optimal barcodes to be multiplexed together based on the total number of samples being combined. If there are additional questions on barcode balance please contact Illumina Tech support.