PyroMark SNP/Allele Quantification Analysis
PyroMark provides real-time sequence information, highly suitable for genetic analysis. It can be used to determine sequence variation–including single nucleotide polymorphisms and insertions/deletions–generating quantifiable results.
|Platform||PyroMark Q96 MD and PyroMark Q96 Vacuum Workstation|
|Primary Contact||Ellen Pedersen
Research Specialist: Sequenom and PyroMark Services
University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||Plate Report (text or Excel file) containing allele quantification percentages and sample/well quality metrics, along with Pyrograms depicting spectra for each well/sample.|
|How to Request||Contact Dr. Lyons for initial consultation; if he deems project appropriate for PyroMark, contact Ellen Pedersen.|
|Sample Requirements||Sample prep according to Qiagen’s specifications; Ellen will arrange direct support from Qiagen to ensure optimization of PCR products prior to sample submission for pyrosequencing.|
|Typical Turnaround||Depends on project queue; each plate/run requires ~4 hours to process.|
|More Information||If Assay Design is completed by Core, the costs will involve additional labor charges billed at the current approved PyroMark Tech time hourly rate, which will be added to the current per plate recharge rate.|