PyroMark Pyrosequencing


PyroMark SNP/Allele Quantification Analysis

PyroMark provides real-time sequence information, highly suitable for genetic analysis. It can be used to determine sequence variation–including single nucleotide polymorphisms and insertions/deletions–generating quantifiable results.

PlatformPyroMark Q96 MD and PyroMark Q96 Vacuum Workstation
Primary ContactEllen Pedersen
Research Specialist: Sequenom and PyroMark Services
University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical OutputPlate Report (text or Excel file) containing allele quantification percentages and sample/well quality metrics, along with Pyrograms depicting spectra for each well/sample.
How to RequestContact Dr. Lyons for initial consultation; if he deems project appropriate for PyroMark, contact Ellen Pedersen.
Sample RequirementsSample prep according to Qiagen’s specifications; Ellen will arrange direct support from Qiagen to ensure optimization of PCR products prior to sample submission for pyrosequencing.
Typical TurnaroundDepends on project queue; each plate/run requires ~4 hours to process.
More InformationIf Assay Design is completed by Core, the costs will involve additional labor charges billed at the current approved PyroMark Tech time hourly rate, which will be added to the current per plate recharge rate.