Epigenomics Cytosine Modifications
Genome-wide DNA Methylation Profiling
The Epigenomics Core offers Genome-wide DNA Methylation Profiling. This sequence-based technology provides information on DNA methylation status across the genome at different levels of resolution:
- Whole-genome bisulfite sequencing: whole-genome base-pair resolution quantification of DNA methylation
- ERRBS (Enhanced Reduced Representation Bisulfite Sequencing): Quantitative base-pair measurement of cytosine methylation at ~3M CpGs genome-wide located at promoters, CpG islands and shores, and intergenic regulatory regions
- meDIP-seq: Antibody-based method for identification of mC-enriched areas across the genome
Targeted (locus-specific) DNA Methylation
Targeted DNA methylation allows for the locus-specific detection of cytosine methylation by bisulfite-sequencing. The Targeted DNA Methylation services at the Epigenomics Core include:
- Primer design consultation and training
- Bisulfite conversion of DNA and PCR amplification of target sequences
- MassARRAY EpiTYPER sequencing
- Pyrosequencing
- Support with data analysis
Hydroxymethylation: Genome-wide and Target Specific
- hmeDIP-seq: Antibody-based method for the identification of 5-hmC-enriched areas across the genome
- hmeDIP: Antibody-based method for the identification of 5-hmC enrichment at specific loci