Epigenomics Cytosine Modifications

Genome-wide DNA Methylation Profiling

The Epigenomics Core offers Genome-wide DNA Methylation Profiling. This sequence-based technology provides information on DNA methylation status across the genome at different levels of resolution:

  • Whole-genome bisulfite sequencing: whole-genome base-pair resolution quantification of DNA methylation
  • ERRBS (Enhanced Reduced Representation Bisulfite Sequencing): Quantitative base-pair measurement of cytosine methylation at ~3M CpGs genome-wide located at promoters, CpG islands and shores, and intergenic regulatory regions
  • meDIP-seq: Antibody-based method for identification of mC-enriched areas across the genome

Targeted (locus-specific) DNA Methylation

Targeted DNA methylation allows for the locus-specific detection of cytosine methylation by bisulfite-sequencing. The Targeted DNA Methylation services at the Epigenomics Core include:

  • Primer design consultation and training
  • Bisulfite conversion of DNA and PCR amplification of target sequences
  • MassARRAY EpiTYPER sequencing
  • Pyrosequencing
  • Support with data analysis

Hydroxymethylation: Genome-wide and Target Specific

  • hmeDIP-seq: Antibody-based method for the identification of 5-hmC-enriched areas across the genome
  • hmeDIP: Antibody-based method for the identification of 5-hmC enrichment at specific loci
Translate »