ChIP-Seq (chromatin immunoprecipitation sequencing) can be used to map genome-wide distribution patterns of specific histone modifications. The Epigenomics Core offers the following ChIP-Seq services:
- Library preparation of user-provided immunoprecipitated DNA
- Immunoprecipitation and library preparation of a selection of human and mouse histone modifications –coming soon!
- ChIP-seq data analysis
At sample submission, the customer must provide the following to the Core:
- At least 10 ng of input and immunoprecipitated DNA for each sample.
- Image of DNA fragmentation QC (gel image or Bioanalyzer/TapeStation results), showing most of fragments between 200-500 bp.
- Percent input enrichment qPCR results (minimum of 4% input enrichment) for both positive and negative controls regions, and IgG control (as excel spreadsheet or table).
- Primers for positive/negative control genes to test fold enrichment post library preparation (diluted to 5uM), preferably with the annealing temperature used by the customer.
Customers submitting samples without providing the preliminary QC data specified above will be charged for library preparation. These requirements are based on the assumption that the customer wants the samples to be processed for library preparation.