How must samples be prepared?
Must samples be filtered prior to running on the core instrumentation?
Do you archive data, and how can I access old data?
Does the core provide Flow Cytometry Software?
Users have two options to purchase software licenses:
- FCS Express annual licenses can be purchased directly from the core. This software may be installed on all of the lab computers with a single concurrent user.
- FlowJo annual licenses can be purchased through the University. However, the core is not directly involved in their sale or distribution. The software license is limited to a single CPU.
Must I schedule an appointment to use Core services?
Yes. The core usually operates with a full-schedule, and experiments are scheduled back-to back. There is no open space currently reserved for Walk-in experiments.
I have a last minute experiment with unexpected samples; can I get them run?
Can I have all of my experiments run by the same operator?
Can I have all of my experiments run on the same instrument?
Can I make scheduling requests via email?
Does the core offer online scheduling?
How can I gain access to the core resources after hours and on weekends?
Please contact David Adams directly for access requirements as they vary by location.
Does the Flow Core offer training on instrumentation?
Yes. The core offers a mandatory monthly training seminar for users wishing to learn to use the core’s equipment. After attending the (no-cost) seminar, the users will be scheduled for hands-on training on the appropriate instrumentation. Hands-on training is done individually, and we will not train multiple users at the same time. As a courtesy, the core will train one user from any given lab at no cost. Additional users are trained at the standard rate for the time used.
I've been doing flow for years, do I really need to attend the seminar?
Do you offer training for both analyzers and sorters?
Yes. However, we are very protective of our sorters, and if we do not feel that you are developing the proper skills, you will not be allowed to use them.
I was trained on the FACSCalibur for free. Do I have to pay for training on additional platforms?
Do you offer training on Flow Cytometry Software?
Not formally, no. However, if the staff is familiar with the piece of software in question, we will be happy to answer any questions we can.
Also, TreeStar offers an annual FlowJo training seminar on campus.
The core contains multiple sorter platforms. Which sorter is best?
Okay. So which is your favorite sorter?
I've thought of a completely novel way to use flow cytometry. Can the core help me?
I'd like to run fluorochrome x or novel fluorescent protein y. Can you purchase a new laser to allow me to do so?
Flow Cytometry Resources
- BDBiosciences MultiColor Flow Cytometry Tools
- Life Technologies Spectra Viewer
- Purdue University Cytometry Mailing List