Light Microscopy

The BRCF Microscopy Core houses a wide range of light microscopes and imaging systems in the following three locations:  BSRB A830, MSII 5631, and NCRC B20 57S. These instruments support a variety of imaging modalities, as described below. Please contact us during the planning stage of your project to help you select the most appropriate methodology for your research needs.

LS
Light Sheet
SR
Super Resolution
MP
Multi Photon
WF
Widefield
SDC
Spinning Disk Confocal
LSM
Laser Scanning Confocal
TIRF
Total Internal Reflection Fluorescence

Instruments Located in BSRB:

LEICA SP8
LSM MP SR
  • A high sensitivity, inverted, point-scanning confocal and multiphoton system, suitable for fixed and live samples.
  • Equipped with a resonant scanner for fast image acquisition.
  • Capable of Fluorescence Lifetime Imaging (FLIM).
  • Single 594 nm depletion line for Stimulated Emission Depletion Microscopy (STED) in XY only.
  • Lasers available for imaging:
    • 405nm diode laser.
    • Argon laser with 5 excitation lines (458nm, 476nm, 488nm, 496nm, 514nm).
    • White light laser tunable from 470nm-670 nm.
    • Ti: Sapphire pulsed IR laser (100 fsec, 80 MHz). Tunable from 670nm-1080nm.

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LEICA SP5
LSM MP
  • A high sensitivity, upright, point-scanning confocal and multiphoton system, equipped with a large stage and dipping lenses. Suitable for live animal imaging, whole-mount samples, and slides.
  • Equipped with a resonant scanner for fast image acquisition.
  • Lasers available for imaging:
    • 405nm Diode laser.
    • Argon Laser with 5 excitation lines (458nm, 476nm, 488nm, 496nm, 514nm)
    • 561nm diode laser
    • 633nm HeNe laser
    • Ti: Sapphire pulsed IR laser (100 fsec, 80 MHz). Tunable from 670nm-1080nm

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LEICA STELLARIS
LSM
  • A high sensitivity, inverted, point-scanning confocal system, suitable for fixed and live samples.
  • Equipped with a resonant scanner for fast image acquisition.
  • Uses Navigator software for easy stitching, tiling, and overview imaging.
  • TauSensing tools, including TauContrast, TauGating and TauSeparation are available. These tools use the differences in fluorescence lifetimes to separate closely overlapping fluorophores and suppress nonspecific background fluorescence.
    • 405nm diode laser.
    • 445nm diode laser.
    • White light laser tunable from 470nm-680 nm.

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ZEISS LATTICE LIGHT SHEET 7
LS
  • Fast and gentle volumetric imaging with minimal photobleaching and phototoxicity.
  • Ideal for long-term imaging of dynamic subcellular processes in living cells. Not recommended for samples greater than 50um thick.
  • 40X objective; 3 laser lines: 488nm, 561nm, and 633nm

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ZEISS LIGHT SHEET 7
LS
  • Gaussian light sheet instrument, ideal for imaging larger format samples including organoids, zebrafish, embryos, and cleared murine organs.
  • Dry and immersion objectives corrected for RI = 1.33 – 1.58; 2X, 5X and 20X objectives.
  • 6 available laser lines: 405nm, 445nm, 488nm, 515nm, 561nm and 647nm.
  • Incubation for live timelapse imaging.

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NIKON A1 HIGH SENSITIVITY CONFOCAL
LSM
  • An inverted point scanning confocal with two standard PMT detectors and two GaAsP detectors.
  • Ideal for slides and other fixed sample formats.  Basic live cell imaging is possible.
  • 4 available laser lines: 405nm, 488nm, 561nm and 647nm

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NIKON A1 STANDARD SENSITIVITY CONFOCAL
LSM
  • An inverted point scanning confocal with standard PMT detectors.
  • Ideal for slides and other fixed sample formats.  Basic live cell imaging is possible.
  • 4 available laser lines: 405nm, 488nm, 561nm and 647nm

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Nikon E800
WF
  • Upright manual widefield microscope for fluorescence, brightfield and polarized light imaging or fixed samples.
  • Color and monochrome cameras are available.
  • Three fluorescence channels for blue, green and orange fluorophores; for example: DAPI, FITC and Rhodamine.

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Zeiss Apotome
WF
  • Upright microscope for confocal-like and epifluorescence imaging of fixed samples.
  • Motorized stage for tiling.
  • Six fluorescence channels for blue, green, orange, red, far-red and near IR fluorophores; for example: DAPI, FITC, Rhodamine, Texas Red, Cy5 and Cy7.

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Olympus SZX16
WF
  • Basic stereomicroscope, capable of brightfield and epifluorescence imaging. Monochrome camera available.
  • Suitable for unmounted samples. Can be used as a dissecting scope.
  • Four fluorescence channels for blue, green, orange, and red fluorophores; for example: DAPI, FITC, Rhodamine and Texas Red.

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Instruments Located in MSII:

NIKON X1 YOKOGAWA SPINNING DISK CONFOCAL
SDC TIRF WF
  • An inverted spinning disk confocal system, ideal for fast, gentle imaging of live samples such as cells in culture and small organoids and organisms. Speeds of 30 fps possible.
  • Digital mirror device for photo-activation or optogenetics using a 395nm or 470nm LED.
  • Through objective TIRF illumination available. Monochrome camera for TIRF and widefield imaging.
  • 4 available laser lines for confocal (3 for TIRF): (405nm), 488nm, 561nm and 647nm.

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NIKON N-SIM + A1R
LSM SR WF
  • An inverted microscope equipped with a point-scanning confocal and a 2D / 3D structured illumination (SIM) system.
  • Equipped with a resonant scanner for fast confocal image acquisition.
  • Timelapse widefield live-cell imaging is also supported.
  • 6 available laser lines for confocal (4 for SIM): 405nm, (445nm), 488nm, (514nm), 561nm and 647nm.

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Instruments Located in NCRC:

NIKON A1SI CONFOCAL
LSM
  • An inverted point scanning confocal, suitable for live and fixed samples.
  • Includes a spectral detector for imaging dyes with highly overlapping emission profiles.
  • 4 available laser lines: 405nm, 488nm, 561nm and 647nm.

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