About the Proteomics Core

Personalized project attention – every step of the way

The Proteomics & Peptide Synthesis Core provides research services in proteomics and custom peptide synthesis. We work with each client to determine the best approach and services needed to fulfill a specific project goal, budget and timeline.

Instrumentation

NEW: We are pleased to now offer services on the Orbitrap Fusion Lumos mass spectrometer, the latest instrument available.  Besides increased resolving power (up to 500,000FWHM), its parallel MS and MSn capability allows for identification of more proteins, more peptides and more post translational modifications.  For quantitative proteomics, this instrument improves accuracy when using isobaric mass tags (SILAC, TMT, iTRAQ).

A picture of the instrumentation available through the Proteomics & Peptide Synthesis Core.

Software Resources

For database searches, localization of post-translational modifications and quantitative analysis we use the following commercially available software packages:

a)   Mascot and Mascot Distiller (Matrix Science LTD, UK)

b)   Scaffold Q+S including X! Tandem  (Proteome Software Inc., Seattle, WA)

A recent inter-laboratory research study conducted among core facilities shows that most laboratories use Mascot and Scaffold platforms. This study was conducted by the ABRF Proteomics Research Group (www.abrf.org) and the results were presented at the 2016 ABRF conference. The publication is currently in preparation.

During data discussion and software training, Dr. Remmer makes sure the client obtains and understands every bit of information relevant to the project goal.