Mass Spectrometry Instrumentation

Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) coupled to a UPLC (Waters)

• Improved accuracy for relative quantitation (TMT, SILAC)
• Identification of more low-level proteins with better accuracy
• Used to analyze highly complex samples
• Sub ppm mass accuracy

Q-Exactive mass spectrometer coupled to UPLC (Waters):

• High throughput screening (post-translational modifications)
• Protein ID
• Label-free quantitation by spectral counting
• Targeted quantitative proteomics (PRM)

The Orbitrap Exploris 4800

• Increased sensitivity and dynamic range
• Used for Data independent acquisition (DIA) and targeted MS/MS (PRM, “absolute” quantitation)

Ultraflextreme MALDI-TOF/TOF mass spectrometer (Bruker):

• Protein intact molecular weight analysis by MALDI-TOF and more