Mass Spectrometry Instrumentation

Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) coupled to a UPLC (Waters)

  • Improved accuracy for relative quantitation (TMT, SILAC)
  • Identification of more low-level proteins with better accuracy
  • Used to analyze highly complex samples
  • Sub ppm mass accuracy


Q-Exactive mass spectrometer coupled to UPLC (Waters):

  • High throughput screening (post-translational modifications)
  • Protein ID
  • Label-free quantitation by spectral counting
  • Targeted quantitative proteomics (PRM)


The Orbitrap Exploris 4800

  • Increased sensitivity and dynamic range
  • Used for Data independent acquisition (DIA) and targeted MS/MS (PRM, “absolute” quantitation)


Ultraflextreme MALDI-TOF/TOF mass spectrometer (Bruker):

  • Protein intact molecular weight analysis by MALDI-TOF and more


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