What We Do
The Transgenic Core routinely uses CRISPR/Cas9 to produce gene-edited mice and rats. The Transgenic Core guarantees that at least three transgenic founder mice or rats will be produced for every transgene DNA construct. Since 1989 the Core has produced more than 20,000 transgenic mouse founders from more than 2,000 transgenes and more than 1,000 transgenic rat founders. Since 2013 the Core has produced over 3,000 transgenic mouse founders from over 300 CRISPR/Cas9 projects, 16 CRISPR/Cas9 mediated rat models have been completed. CRISPR/Cas9 technology was used for gene knockouts, codon changes, epitope tags, floxed genes, reporter knockins (EGFP, Cre recombinase, etc.), ROSA26 knockins, and specialized projects (regulatory sequence knockouts, ES cell engineering, etc.). Over 200 mouse strains have been produced for genes targeted in ES cells in the Core and another 60 strains from ES cells obtained from the International Knockout Mouse Consortium. In addition, the Transgenic Core offers a full suite of cryopreservation and assisted reproduction (IVF) services.
The efficiency of transgenic mouse and rat model production meets or exceeds standards in the published literature.