Transgenic Core Cost Estimates & Fees
Fees below are for internal customers only. If you are an external customer, contact the core for pricing. Please see our Services page for more information on available services.
| Service Offered | Cost | Description |
|---|---|---|
| B6SJLF2 Hybrid Transgenic Mouse Production | $3,700.00 | The Transgenic Core guarantees that at least 3 transgenic founders will be produced from your transgene DNA |
| C57BL/6J Transgenic Mouse Production | $5,800.00 | The Transgenic Core guarantees that at least 3 transgenic founders will be produced from your transgene DNA |
| Custom Genetic Background Transgenic Mouse Production | $6,800.00 | The Transgenic Core guarantees that at least 3 transgenic founders will be produced from your transgene DNA; The investigator will pay shipping and per diems costs for custom donor strains |
| Sprague Dawley Outbred Transgenic Rat Production (other strains available) | $11,000.00 | The Transgenic Core guarantees that at least 3 transgenic founders will be produced from your plasmid transgene DNA. The Core will purify DNA for microinjection from restriction enzyme digested plasmid DNA provided by the Investigator. The investigator will pay shipping and per diems costs for custom donor strains |
| CRISPR/Cas9 Reagents | $1,100.00 | The Transgenic Core will build CRISPR/Cas9 reagents to target a specific location in the mouse or rat genome. |
| CRISPR/Cas9 Zygote Microinjection Test | $390.00 | The Transgenic Core will microinject CRISPR/Cas9 reagents into 30 fertilized mouse eggs and culture surviving eggs to the blastocyst stage (~60 cells). The blastocysts will be placed in PCR tubes provided by the investigator for analysis of Cas9 activity in the investigator's laboratory. |
| B6SJLF2 Hybrid CRISPR Modified Mice | $3,700.00 | The Transgenic Core guarantees that at least 300 fertilized mouse eggs will be microinjected with CRISPR/Cas9 reagents. Microinjected eggs will be transferred to pseudoopregnant female mice. Tail tip biopsies will be provided to the Investigator's laboratory for genotyping. Mouse pups will be transferred to the investigator at weaning. |
| C57BL/6J CRISPR Modified Mice | $5,800.00 | The Transgenic Core guarantees that at least 300 fertilized mouse eggs will be microinjected with CRISPR/Cas9 reagents. Microinjected eggs will be transferred to pseudoopregnant female mice. Tail tip biopsies will be provided to the Investigator's laboratory for genotyping. Mouse pups will be transferred to the investigator at weaning. |
| Sprague Dawley CRISPR Modified Rats (other strains available) | $11,000.00 | The Transgenic Core guarantees that at least 300 fertilized rat eggs will be microinjected with CRISPR/Cas9 reagents. Microinjected eggs will be transferred to pseudoopregnant female rats Tail tip biopsies will be provided to the Investigator's laboratory for genotyping. Rat pups will be transferred to the investigator at weaning. |
| Mouse Sperm Cryopreservation | $1,100.00 | Mouse sperm is cryopreserved and stored in liquid nitrogen for recovery of live mice at a future date. |
| Mouse in vitro Fertilization with fresh or frozen sperm | $2,500.00 | in vitro fertilization will be used to produce large numbers of live mice from live male sperm donors OR Recovery of live mice from cryopreserved mouse sperm by in vitro fertilization. |
| Mouse 2-Cell Egg Cryopreservation | $1,800.00 | Mouse embryos (2-cell or 8-cell eggs) are cryopreserved and stored in liquid nitrogen for recovery of live mice at a future date. |
| Cryopreserved Mouse Egg Thaw and Line Recovery | $1,300.00 | Recovery of live mice from cryopreserved mouse embryos. |
| Rat Egg Cryopreservation | $3,000.00 | Rat egg donors and sperm donors will be used to produce fertilized eggs that can then be used to cryopreserve the rat line. |
| Liquid Nitrogen sample storage (annual fee) | $450.00 | Cryopreserved mouse embryos (2-cell or 8-cell eggs) or cryopreserved mouse sperm are stored in liquid nitrogen for a period of 3 years. |
| Rederivation of Pathogen Free Mouse Lines | $1,200.00 | Pathogen Free Rederivation of Infected Mice |
| CRISPR/Cas9 - ES Cell Training Class | $1,900.00 | The purpose of the class is to provide training in the use of CRISPR/Cas9 technology and to provide the scientific background needed to make genetically engineered mouse models, rat models or engineered mouse ES cells. You will learn both methods and the principles behind the technology. You will be trained in all aspects of ES cell culture manipulation and the scientific background needed to produce gene targeted ES cell lines and mouse models. You will learn the methods and the principles of mouse genome engineering technology. |
| Pronuclear Microinjection Training | $5,200.00 | Hands-on training in the pronuclear microinjection of fertilized eggs to produce transgenic founder animals. It is common for trainees to successfully produce founders. |
| ES Cell Electroporation (five 96-well plates of ES cell clones) | $5,300.00 | The Transgenic Core will recover live cell cultures from ES cell clones stored in frozen 96-well plates and characterize them. |
| ES Clone Expansion | $1,300.00 | The Transgenic Core will place ES cell clones in culture. The cells will be cultured until several vials can be cryopreserved. The investigator will receive a cell pellet from which DNA or RNA can be extracted for verifcation of correct gene targeting in the cells. Twenty chromosome spreads will be counted and the modal chromosome number will be used to determine if the cells are euploid. The cells will be tested for mycoplasma contamination and assessed for gross differentiation . |
| Blastocyst Microinjection of ES cells | $4,600.00 | The Transgenic Core guarantees that at least 50 blastocysts will be microinjected with ES cells to produce ES cell-mouse chimeras for the investigator. |
| Bacterial Artificial Chromosome Recombineering | $3,800.00 | Recombineering is the process by which BACs are modified. The resulting BAC transgenes can be used to produce transgenic mice, rats, or cell lines. |
| Bacterial Artificial Chromosome DNA Purification | $360.00 | BAC DNA will be purified from a bacterial stock. BAC DNA quality will be evaluated by pulsed field gel electrophoresis and restriction enzyme mapping. |
| Sperm ICSI | $1400.00 | IntraCytoplasmic Sperm Insertion (ICSI) in mouse oocytes is used to recover a mouse line from sperm when standard IVF methods are unsuccessful. |
| Mycoplasma Test | $180.00 | Media from cultured cells is evaluated for the presence of mycoplasma with the Lonza MycoAlert Mycoplasma Detection Kit. |
Subsidies on Services are Available to Members of University of Michigan Research Centers