Transgenic Animal Model Products & Services Services Description Transgenic Core CRISPR/Cas9 Pipeline Material Transfer Agreements Transgenic Rats BAC Recombineering Core Requests Training & Education Laboratory Protocols Genotyping Sample Submission Instructions Transgenic Mouse and Transgenic Rat Outline Gene Targeting Outline Mouse Breeding Cost Estimates & Fees FAQ Pertinent Publications Supported Publications About Laboratory Protocols h DNA Purification for Microinjection h BAC DNA Purification for Microinjection h DNA Purification for Electroporation h Preparation of DNA from Tail Biopsies h PCR Analysis of Tail DNA h Transgene Copy Standards h Mouse Blastocyst PCR Assay h RNA Microinjection Buffer and Cas9 mRNA Reagents h In Vitro and In Vivo Assay for CRISPR/Cas9 h Cel I Assay to Detect Non-Homologous Endjoining Repair h CRISPR/Cas9 ES Cell Electroporation h Mouse beta globin Primers for PCR h RAT prolactin Primers for PCR h Neo Primer for PCR h Hot Shot DNA PCR Assay h lacZ Primer for PCR h lacZ Tissue Staining h Timeline for Transgenic Mouse Production/Analysis h Timeline for Gene Targeted Mouse Production/Analysis h ES Cell Chromosome Counting
