Laboratory Protocols

DNA Purification for Microinjection
BAC DNA Purification for Microinjection
DNA Purification for Electroporation
Preparation of DNA from Tail Biopsies
PCR Analysis of Tail DNA
Transgene Copy Standards
Mouse Blastocyst PCR Assay
RNA Microinjection Buffer and Cas9 mRNA Reagents
In Vitro and In Vivo Assay for CRISPR/Cas9
Cel I Assay to Detect Non-Homologous Endjoining Repair
CRISPR/Cas9 ES Cell Electroporation
Mouse beta globin Primers for PCR
RAT prolactin Primers for PCR
Neo Primer for PCR
Hot Shot DNA PCR Assay
lacZ Primer for PCR
lacZ Tissue Staining
Timeline for Transgenic Mouse Production/Analysis
Timeline for Gene Targeted Mouse Production/Analysis
ES Cell Chromosome Counting