Vector Core Cost Estimates and Fees
For a full list of products and services, please visit the Products & Services page.
Adenovirus
| Service | Rate* (Internal) | Rate (External) | Turn-Around Time | Notes |
| Adenoviral – Construction with Plaque Amplification | $1,031.00 | $1,546.50 | 4 weeks for construction (minimum). For external users: Additional time needed for amplification (2 weeks) and titering (2 weeks). | For each construction, 50 μg of shuttle DNA (purified and completely cut by either PmeI or NheI) is required. For custom construction of commercial vector systems that are already full length (such as AdenoX, Adeasy), please linearize the plasmid according to the system specifications. |
| Adenoviral – Large Scale Purification and Expansion | $1,681.00 | $2,521.50 | 2 weeks | Need 100 μl of lysate or 10 μl purified. Make sure the virus has not been serially propagated. |
| Clonal Isolation and Plaque Amplification | $454.00 | $681.50 | Up to 12, 1 ml amplified clones of adenovirus (primary lysate). | |
| Adenoviral – Standard Glycerol Stock | $295.00 | $442.50 | same day | Pre-made stocks of popular viral vectors at 1012 particles. Titer is included. Each sample is 0.25 mls. Store at -80°C. |
| Adenoviral – Titer (plaque assay) | $354.00 | $531.00 | 3 weeks | This requires at least 20 μl of purified virus. |
| Adenoviral – Crude Lysate, 50 mls | $245.00 | $367.50 | 2 weeks | The cell debris is removed, but Crude Lysate is NOT purified or concentrated. |
Adeno-Associated Virus (AAV)
| Service | Rate* (Internal) | Rate (External) | Turn-Around Time | Notes |
| Adeno-Associated Virus tester scale prep (50 ul of ~1×1013 vg/ml virus) | $987.00 | $1,480.50 | 2 weeks | We offer serotypes: AAV-1, -2, -5, -6, -7, -8, -9, -PHP.B, -PHP.eB, -PHP.S, -Retro (*if you need a different serotype than the ones listed, please inquire). Each tester scale AAV prep requires 25μg of purified DNA. |
| Adeno-Associated Virus Small scale prep (300 ul of ~1×1013 vg/ml virus) | $1,533.00 | $2,299.50 | 2 weeks | We offer serotypes: AAV-1, -2, -5, -6, -7, -8, -9, -PHP.B, -PHP.eB, -PHP.S, -Retro (*if you need a different serotype than the ones listed, please inquire). Each small scale AAV prep requires 130μg of purified DNA. |
| Adeno-Associated Virus Large scale prep (1000 ul of ~1×1013 vg/ml virus) | $2,647.00 | $3,970.50 | 2 weeks | We offer serotypes: AAV-1, -2, -5, -6, -7, -8, -9, -PHP.B, -PHP.eB, -PHP.S, -Retro (*if you need a different serotype than the ones listed, please inquire). Each large scale AAV prep requires 400μg of purified DNA. |
| Adeno-Associated Virus Pre-made Stock (50 ul of 1×1013 – 1×1014 vg/ml virus) | $248.00 | $372.00 | same day | Currently we offer GFP, mCherry, and CRE driven by several different promoters packaged in several serotypes; please inquire. We also have Brainbow (rAAVPHPeB-EF1a-BbTagBY and rAAVPHPeB-EF1a-BbChT). We are making new stocks all the time, so check with us or provide input on viruses to stock. |
| Adeno-Associated Virus Titering Service | $202.00 | $283.00 | For titering up to 5 Adeno-Associated Viruses. |
Lentivirus/Retrovirus
| Service | Rate* (Internal) | Rate (External) | Turn-Around Time | Notes |
| Lentiviral shRNA tester samples (5 × 3 ml of 1X virus in DMEM+10%FBS) | $309.00 | $463.50 | 2 weeks | PLEASE note the backbone of each construct, so the Vector Core can check the packaging generation. Each 3 ml tester prep requires 2.5 μg of purified proviral plasmid DNA. |
| Lentiviral/Retroviral – Concentrated Small Scale Prep (10 mls of 10X virus in DMEM or as requested) | $678.00 | $1,017.00 | 2 weeks | PLEASE note the backbone of each construct on the order form, so the Vector Core can check the packaging generation. Each small scale prep requires 70 μg of purified proviral plasmid DNA. This retroviral service requires the VSVG envelope. |
| Lentiviral/Retroviral – Concentrated Large Scale Prep (100 mls of 10X virus in DMEM or as requested) | $1,627.00 | $2,440.50/td>
| 2 weeks |
PLEASE note the backbone of each construct on the order form, so the Vector Core can check the packaging generation. Each large scale prep requires 650 μg of purified proviral plasmid DNA. This retroviral service requires the VSVG envelope. For in-vivo work, 100X concentration is typical. |
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| Lentivirus – Pre-made Standard Virus Stock (10 mls at 10X in DMEM) | $366.00 | $549.00 | same day | Pre-made stocks of popular lentiviral vectors at 107 TU/ml (pLL3.7dsRED, pLLEV GFP, pLLRSV GFP, pLLEV Luc, pGipZ Scrambled). Store at -80°C. |
| Retrovirus – UNCONCENTRATED Small Scale Prep (100 mls of 1X virus). For ecotropic and amphotropic envelope pseudotypes. | $564.00 | $846.00 | 2 weeks | For use with MMLV/MSCV plasmids pseudotyped with ecotropic and amphotropic envelopes which are too fragile to concentrate without great loss. The unconcentrated small scale prep requires 100 μg of proviral DNA. |
| Lentiviral/Retroviral Transduction | $310.00 | $465.00 | 1 week | Transduction of lentiviral constructs onto cells provided by the customer, or on A549 lung carcinoma cells provided by the Vector Core. |
| Lentiviral/Retroviral Titer with Fluorescence | $369.00 | $553.50 | 1-2 weeks | 200 μl of virus is required. |
| Lentiviral/Retroviral Titer with Antibiotic Selection | $417.00 | $625.50 | 2-3 weeks | 200 μl of virus is required. |
shRNA Library Clones
| Service | Rate* (Internal) | Rate (External) | Turn-Around Time | Notes |
| In-Stock shRNA Library Clones | $35.00 per clone (if available in our library) | Not Available | 1-2 days for in-stock clones | The Vector Core currently stocks all pGipZ V2L and a few other lentiviral shRNA/shRNAmir clones. Please click the shRNA services link and go to shRNA Library Clones. (shRNA Services Link) |
| Non-Stock pLKO Clones | $55.00 per clone /td> | Not Available | Varies | The Vector Core currently stocks pLKO clone ordering through Sigma for any clones that we do not currently carry in our stocks. |
Plasmids
| Service | Rate* (Internal) | Rate (External) | Turn-Around Time |
| Plasmid Midi Prep (~100 μg) | $91.00 | $136.50 | 1 week |
| Plasmid Maxi Prep (~0.5 mg) | $160.00 | $240.00 | 1 week |
| Plasmid Mega Prep (~1mg) | $298.00 | $447.00 | 1 week |
| Plasmid Giga Prep (~5 mg) | $613.00 | $919.50 | 1-2 weeks |
| Plasmid DNA Transformation into Bacteria | $95.00 | $142.00 | 1 week |
Consultation
| Service | Rate* (Internal) | Rate (External) | Turn-Around Time | Notes |
| 1-hour consultation | $51 | $70 | 1 week to schedule appointment time | Having trouble planning/executing your project? Consult with the Vector Core manager Tom Lanigan for advice on how to use our vectors. |
| Custom Services | Inquire | Inquire | Do you require a service that is not listed on our order forms? E-mail Tom Lanigan, lanigant@umich.edu with what you require and we will customize a solution for you! |