Vector Products and Services

Adeno-Associated Virus (AAV): AAV provides broad host range and long-term expression in vivo. The Core now offers a wide-range of AAV serotypes and will offer several scales of viral production.

Adenovirus provides high expression, transient transfection to a wide variety of dividing and non-dividing cell types. Adenovirus is a widely used viral transfection vector for in vivo and in vitro applications.

Lentivirus is a retrovirus that provides stable integration in both dividing and nondividing cells. Lentivirus is used for the production of permanent cell lines and long-term modifications for in vivo and ex vivo applications.

Retrovirus (MMLV/MSCV) provides stable integration in dividing cells. Retrovirus is used for the production of permanent cell lines and long-term modifications for in vivo and ex vivo applications.

Plasmid services, including adenoviral shuttle plasmids, retroviral plasmids, lentiviral plasmids, CRISPR/Cas9 genome editing plasmids and pUMVCexpression plasmids.

shRNA library clones: The Vector Core currently stocks all GE Dharmacon pGIPZ V2L (Oligo ID #) clones and a selection of lentiviral miRNA/shRNA clones in the pGIPZ V3L, pTRIPZ, and pLKO backbones. Non-stock lentiviral clones (Sigma pLKO clones) will be purchased on request for $50/clone.

CRISPR/Cas9 genome editing plasmids: The Vector Core offers consultation on targeted genome editing technologies to U-M investigators. In collaboration with Sigma, the Vector Core offers special pricing on Sigma’s CRISPR/Cas9 all-in-one (multiple platforms) and ready-to-use Cas9 plasmids as well as guide RNA (gRNA).