Adeno-Associated Virus (AAV)

AAV Custom / Stock Services

Serotypes available:  1, 2, 5, 6, 7, 8, 9, rh10, hu11, rh32.33,  PHP.B/eB/S, and Retro.  (*If you need a different serotype from those listed, please inquire.)

 

Service Scale DNA amount required* Yield Internal Price External Price
Tester Scale 25 ug 50 ul,       ~1×10^13 vg/ml $866.00 $1,299.00
Small Scale 150 ug 300 ul,     ~1×10^13 vg/ml $1412.00 $2,118.00
Large Scale 400 ug 1000 ul,   ~1×10^13 vg/ml $2474.00 $3,464.00
Stocks N/A **50/100 ul,     ~1×10^13 vg/ml $221.00 $309.00
Stock Serotype Sampler (CAG-eGFP) N/A 20 ul, (1,2,5,8,9) ~1×10^13 vg/ml inquire
Adeno-Associated Virus Titering Service N/A Titering of up to 5 Adeno-Associated Viruses $202.00 $283.00

 *DNA quality is critical for the best viral titer! (verify your DNA by enzymatic digestion on a gel)

** WE ARE NOW PRODUCING STOCKS IN 100 UL ALIQUOTS, HOWEVER SOME PREVIOUSLY PRODUCED STOCKS WILL BE 50 UL. THE 50 UL AND 100 UL STOCKS HAVE THE SAME NUMBER OF VIRAL GENOMES (VG), JUST DIFFERENT CONCENTRATIONS. 

AAV Stocks Available

CRE

Transgene Serotype(s)
AAV_TBG_CRE 8
AAV-CMV-GFP/CRE 5, 8
AAV-CAMKII-CRE 9
AAV-hSyn-CRE 8, 9

GFP

Transgene Serotype(s)
AAV-CAMKII-eGFP 9, PhP.eb
AAV-EF1a-eGFP 8
AAV-hSyn-eGFP 8, PhP.eb
AAV-TBG-eGFP 8
AAV-EF1a-DIO-EYFP 8
AAV-CAG-eGFP 1, 2, 5, 8, 9, PhP.eb
AAV-CAG-eGFP (sampler pack) 1, 2, 5, 8, 9

mCherry

Transgene Serotype(s)
AAV-Efc-mCherry 2, 8, 9
AAV-CAG-mCherry PhP.eb
AAV-EF1a-DIO-mCherry 8

Channelrhodopsin

Transgene Serotype(s)
EF1a-DIO-hChR2(H134R)-EYFP 8

GCAMP

Transgene Serotype(s)

Controls

Transgene Serotype(s)

Misc

Transgene Serotype(s)

ShRNA

Transgene Serotype(s)

DREADDs

Transgene Serotype(s)

AAV Custom Cloning Backbones

This section is currently under construction.

AAV Useful Information

What is AAV:

AAV (adeno-associated virus) is a viral vector system that is often used to effectively transduce large numbers of cells in vivo.

Why Choose AAV:

Recombinant AAV has a broad host range with long-term expression in vivo, and low immunogenicity. AAV offers several distinct advantages to other viral vector systems in that:

  1. Tropism is determined by choice of capsid serotype (see table below, also see addgene’s AAV Data hub).
  2. Long-term expression can be seen in terminally differentiated cells.
  3. A wide-range and large numbers of cells can be infected, including non-dividing cells.
  4. Note: AAV transgenes are limited to < 4.7 kb in size. Larger transgenes are not efficiently packaged, resulting in lower titers.

AAV Tropism:

The following table provides a general guide for determining which serotype to use for your research.

 From VectorBuilder website

Tissue type Recommended AAV serotypes
Smooth muscle AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10
CNS AAV1, AAV2, AAV4, AAV5, AAV7, AAV8, AAV9, AAVrh10, AAV-PHP.eB
PNS AAV-PHP.S
Brain AAV1, AAV2, AAV5, AAV7, AAV8, AAV-DJ/8
Retina AAV1, AAV2, AAV4, AAV5, AAV7, AAV8, AAV9, AAVrh10, AAV2.7m8
Inner ear AAV1, AAV2, AAV6.2, AAV8, AAV9, AAV2.7m8
Lung AAV1, AAV3, AAV4, AAV5, AAV6, AAV6.2, AAV9, AAVrh10
Liver AAV1, AAV2, AAV3, AAV6, AAV6.2, AAV7, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8
Pancreas AAV1, AAV2, AAV6, AAV8, AAV9, AAVrh10
Heart AAV1,AAV4, AAV5, AAV6, AAV8, AAV9, AAVrh10, AAV-DJ
Kidney AAV2, AAV4, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8
Adipose AAV6, AAV8, AAV9
Testes AAV2, AAV9
Spleen AAV-DJ, AAV-DJ/8
Spinal nerves AAV2-retro
Endothelial cells AAV2-QuadYF

MTA Information

For more information regarding MTA, please contact Vector Core Managing Director, Tom Lanigan.

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