Lentivirus

LENTIVIRUS

Lentiviral Services
Service Yield/Amount Internal Price** External Price
Lentiviral shRNA tester samples (5 × 3 ml of supernatant). Tester samples are a “quick and dirty” product tailored toward screening several shRNA products. Each 3 ml tester prep requires  2.5 μg of purified proviral plasmid DNA. The cost is for a set of five. However, if you do not have 5 constructs, multiple 3 ml preps of the same construct can be performed (2 of one construct and 3 of another, etc.).

$240

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Concentrated Lentiviral/Retroviral Production   (small scale, VSVG) 100 mls of supernatant concentrated to 10 mls of 10X (or as requested) in DMEM (or as requested); 80 μg of proviral plasmid required. $540 $835
Concentrated Lentiviral/Retroviral Production   (large scale, VSVG) 1 liter of supernatant concentrated to 100 mls of 10X (or as requested) in DMEM (or as requested); 650 μg of proviral plasmid required. $1,260 $1,850
Concentrated Lentiviral/Retroviral Stock   (10 ml at 10X concentration) Pre-made stocks of popular viral vectors at 107 TU/ml (pLL3.7dsRED, pLLEV GFP, pLLRSV GFP, pLLEV Luc, pGipZ Scrambled). Each sample is 10 mls of 10X concentrated virus in DMEM. $270

$420

Lentiviral/Retroviral Titer with Fluorescence   200 μl of virus is required. 1-2 weeks

$215

$335

Lentiviral/Retroviral Titer with Antibiotic Selection   200 μl of virus is required. 2-3 weeks

$320

$495

Lentiviral/Retroviral Transduction Transduction of lentiviral constructs onto cells provided by the customer, or on A549 lung carcinoma cells provided by the Vector Core.

$130

$200

* Standard turn-around time is 2 weeks for lentiviral services unless otherwise noted.

** Discounts may be available for members of Centers that support the Vector Core (Cancer – 30%; GI Peptide – 50%).

Lentivirus (Ready-Made Stock)
Name Map Sequence Species Description
Lenti-GFP
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 rHIV Reporter vector that allows experimental model infection conditions to be optimized through a GFP marker. Expression is driven from a CMV promoter. The proviral plasmid is pLLEV-GFP.
Lenti-Luc
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rHIV Reporter vector expressing a luciferase marker to allow experimental model gene transfer optimization. Expression driven from a CMV promoter. The proviral plasmid is pLLEV-Luc.
Lenti-RSV-GFP
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rHIV Model control for pLL3.7 dsRed in which RSV promoter (empty) used to drive expression of trans-gene. The GFP marker contained in the vector is expressed through a separate CMV promoter downstream of the RSV expression cassette. The proviral plasmid is pLL RSV-GFP.
Lenti 3.7 dsRED
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rHIV Model control for pLL3.7 dsRed in which U6 promoter (empty) was used to drive expression of shRNA. The dsRed marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. The proviral plasmid is pLL 3.7-dsRED.
Lenti-pGipZ scrambled control
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rHIV Model control for pGipZ constructs containing an shRNAmir. The construct has a CMV promoter with GFP-IRES-Puro-shRNAmir expression cassette. The proviral plasmid is pGipZ scrambled control (non-silencing). The non-silencing shRNAmir sequence is expressed under the control of the CMV promoter and contains no homology to known mammalian genes.
Lentiviral Plasmids
Name Map Sequence Species Description
pLentilox 3.7 GFP
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rHIV Proviral plasmid used in shRNA construction in which shRNA expression is driven through a U6 promoter. GFP marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. This allows simultaneous expression of both shRNA and fluorescent marker. GFP is co-integrated into host cell genome with shRNA which allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox 3.7 dsRed
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rHIV Proviral plasmid used in shRNA construction in which shRNA expression is driven through a U6 promoter. The dsRed marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. This allows simultaneous expression of both shRNA and selectable marker. dsRed is co-integrated into host cell genome with shRNA which allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox 3.7 Puro
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rHIV Pro-viral plasmid used in shRNA construction containing a puromycin selectable marker. U6 promoter is upstream of CMV-Puromycin expression cassette for simultaneous production of shRNA and selectable marker. Puromycin co-integrates into host cells genome which allows for convenient, stable selection.

pLentilox RSV GFP

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rHIV Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for GFP marker utilizing a CMV promoter. GFP is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox RSV2dsRed
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rHIV Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for dsRed marker utilizing a CMV promoter. dsRed is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox RSV puro
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rHIV Transgene expression of this pro-viral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for puro marker utilizing a CMV promoter. Puro is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and puromycin selection.
pLentilox IRES GFP
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rHIV Proviral plasmid containing a bicistronic element to express both a transgene and GFP marker driven by one CMV promoter through the use of an IRES element. IRES elements tend not to be as strong as typical promoter expression cassettes and therefore could result in a weaker GFP signal.
 pLentilox IRES Puro
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rHIV Proviral plasmid containing a bicistronic element to express both a transgene and a puromycin selectable marker driven by one CMV promoter through the use of an IRES element. IRES elements tend not to be as strong as typical promoter expression cassettes and therefore could result in a weaker puromycin response.

pLentilox EV

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rHIV Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains no selectable marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.

pLenti6/V5-DEST

N/A N/A rHIV This Invitrogen expression system utilizes the Gateway Technology of gene transfer. This vector is ideal for multiple expression systems. Contains blasticidin resistance gene for stable selection. This plasmid is fully compatible with Vector Core system for custom lentivirus production.
pLenti6/V5-GW/LacZ N/A N/A rHIV This Invitrogen expression system utilizes the Gateway Technology of gene transfer. This vector allows simple subcloning of transgene. Blasticidin resistance gene for stable selection. This plasmid is fully compatible with Vector Core system for custom lentivirus production.
pLentilox EV-GFP
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rHIV Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains the GFP marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.
pLentilox EV-Luc
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rHIV Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains the luciferase (firefly) marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.
Lenti-FIV-GFP N/A N/A

rFIV

Recombinant Feline Immunodeficiency Virus (FIV) reporter vector expressing the GFP marker. Expression is driven from an RSV promoter.
Lenti-FIV-LacZ N/A N/A

rFIV

Recombinant Feline Immunodeficiency Virus (FIV) reporter vector expressing the β-gal marker. Expression is driven from an RSV promoter.
Lentiviral shRNA Library Clones
Please see our shRNA page for details.