Adenovirus Plasmids Available For Purchase

Name	Map	Sequence	Species	Description
pLentilox 3.7 GFP			rHIV	Proviral plasmid used in shRNA construction in which shRNA expression is driven through a U6 promoter. GFP marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. This allows simultaneous expression of both shRNA and fluorescent marker. GFP is co-integrated into host cell genome with shRNA which allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox 3.7 dsRed			rHIV	Proviral plasmid used in shRNA construction in which shRNA expression is driven through a U6 promoter. The dsRed marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. This allows simultaneous expression of both shRNA and selectable marker. dsRed is co-integrated into host cell genome with shRNA which allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox 3.7 Puro			rHIV	Pro-viral plasmid used in shRNA construction containing a puromycin selectable marker. U6 promoter is upstream of CMV-Puromycin expression cassette for simultaneous production of shRNA and selectable marker. Puromycin co-integrates into host cells genome which allows for convenient, stable selection.
pLentilox RSV GFP			rHIV	Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for GFP marker utilizing a CMV promoter. GFP is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox RSV2dsRed			rHIV	Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for dsRed marker utilizing a CMV promoter. dsRed is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox RSV puro			rHIV	Transgene expression of this pro-viral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for puro marker utilizing a CMV promoter. Puro is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and puromycin selection.
pLentilox IRES GFP			rHIV	Proviral plasmid containing a bicistronic element to express both a transgene and GFP marker driven by one CMV promoter through the use of an IRES element. IRES elements tend not to be as strong as typical promoter expression cassettes and therefore could result in a weaker GFP signal.
pLentilox IRES Puro			rHIV	Proviral plasmid containing a bicistronic element to express both a transgene and a puromycin selectable marker driven by one CMV promoter through the use of an IRES element. IRES elements tend not to be as strong as typical promoter expression cassettes and therefore could result in a weaker puromycin response.
pLentilox EV			rHIV	Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains no selectable marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.
pLenti6/V5-DEST	N/A		rHIV	This Invitrogen expression system utilizes the Gateway Technology of gene transfer. This vector is ideal for multiple expression systems. Contains blasticidin resistance gene for stable selection. This plasmid is fully compatible with Vector Core system for custom lentivirus production.
pLenti6/V5-GW/LacZ	N/A		rHIV	This Invitrogen expression system utilizes the Gateway Technology of gene transfer. This vector allows simple subcloning of transgene. Blasticidin resistance gene for stable selection. This plasmid is fully compatible with Vector Core system for custom lentivirus production.
pLentilox EV-GFP	N/A		rHIV	Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains the GFP marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.
pLentilox EV-Luc	N/A		rHIV	Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains the luciferase (firefly) marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.
Lenti-FIV-GFP	N/A	N/A	rFIV	Recombinant Feline Immunodeficiency Virus (FIV) reporter vector expressing the GFP marker. Expression is driven from an RSV promoter.
Lenti-FIV-LacZ	N/A	N/A	rFIV	Recombinant Feline Immunodeficiency Virus (FIV) reporter vector expressing the β-gal marker. Expression is driven from an RSV promoter.

***Non-University of Michigan customers: please contact Aldevron to purchase stock pUMVC expression plasmids.

Note to all Vector Core customers: the pNGVL plasmids have been renamed as pUMVC. The plasmids are still available to all investigators, but the reference name has changed. If you have any questions please contact the lab supervisor Tom Lanigan at lanigant@umich.edu.