Plasmids

Plasmid Services

DNA Plasmid Services

The University of Michigan Core offers DNA plasmid preparation services. Plasmid preparation services, expected DNA yields and cost are listed in the table below. Typical turn around time is 7 days.

Service Yield/Amount Internal Price External Price
Plasmid Midiprep (~100 μg) Custom purified DNA prep, where the yield is approximately 100 μg. $88.00 $123.00
Plasmid Maxiprep (~500 μg) Custom purified DNA prep, where the yield is approximately 500 μg. $151.00 $211.00
Plasmid Megaprep (~1000 μg) Custom purified DNA prep, where the yield is approximately 2000 μg. $288.00 $403.00
Plasmid Gigaprep (~5000 μg) Custom purified DNA prep, where the yield is approximately 5000 μg. $605.00 $847.00

In addition to the DNA plasmid preparation services the Vector core also has a variety of adenovirus, retrovirus/lentivirus, and protein expression plasmids available for purchase. This is in addition to lentiviral shRNA clones available for purchase from our shRNA mouse and human DNA repository (click here for more information on shRNA repository).

Service Internal Price External Price
Stock Plasmid (10 μg) Tube of 10 μg of purified plasmid $64.00 $100.00**
In Stock shRNA Plasmids Provided as a bacterial streak $30.00 N/A

Adenoviral Shuttle Plasmids

Name Map Sequence Description
pACCMV2
Adenoviral shuttle plasmid with CMV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and unique restriction tag SwaI, PmeI, NheI for linearization. Updated version of pACCMVpLpA(-)loxp-SSP. Shuttle used to produce Ad5 E1/E3-deleted virus.
pACRSV2
Adenoviral shuttle plasmid with RSV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and unique restriction tag SwaI, PmeI, NheI for linearization. Updated version of pARSVpLpA(-)loxp-SSP. Shuttle used to produce Ad5 E1/E3-deleted virus.
pAdEF1 alpha loxP
 Adenoviral shuttle plasmid with EF1 alpha promoter, polylinker, bovine growth hormone polyA site, loxP and unique restriction site NheI for linearization. Shuttle used to produce Ad5 E1/E3-deleted virus.
pACpL+loxP-SSP
Adenoviral shuttle plasmid with pUC19 polylinker and unique restriction tag SwaI, PmeI, NheI for linearization. Does not contain promoter or polyA site. Shuttle used to produce Ad5 E1/E3-deleted virus.
pACCMVpLpA(-) loxP-SSP
Adenoviral shuttle plasmid with CMV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and unique restriction tag SwaI, PmeI, NheI for linearization. Shuttle used to produce Ad5 E1/E3-deleted virus.
pACRSVpLpA(-) loxP-SSP
 Adenoviral shuttle plasmid with RSV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and unique restriction tag SwaI, PmeI, NheI for linearization. Shuttle used to produce Ad5 E1/E3-deleted virus.
pACCMVpLmP1 (-)loxP-SSP
 Adenoviral shuttle plasmid with CMV promoter, pUC19 polylinker, mP1 splicing signal/poly A, loxP with unique restriction tag SwaI, SfiI, PmeI for linearization. Shuttle used to produce Ad5 E1/E3-deleted virus.

Retroviral Plasmids

Name Map Sequence Description

pRET10

Proviral plasmid used in the construction of recombinant MoMLV. This plasmid contains a bicistronic element to express both a transgene and GFP marker driven by one CMV promoter through the use of an IRES element. The CMV promoter is followed by an intron region flanked with donor and acceptor splicing sites before the multi-splicing site.

pRET6

Proviral plasmid used in the construction of recombinant MoMLV. This plasmid contains a bicistronic element to express both a transgene and puromycin selectable marker driven by one CMV promoter through the use of an IRES element. The CMV promoter is followed by an intron region flanked with donor and acceptor splicing sites before the multi-splicing site.

 

Lentiviral Plasmids

Name Map Sequence Species Description
pLentilox 3.7 GFP   rHIV Proviral plasmid used in shRNA construction in which shRNA expression is driven through a U6 promoter. GFP marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. This allows simultaneous expression of both shRNA and fluorescent marker. GFP is co-integrated into host cell genome with shRNA which allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox 3.7 dsRed
rHIV Proviral plasmid used in shRNA construction in which shRNA expression is driven through a U6 promoter. The dsRed marker contained in the vector is expressed through a separate CMV promoter downstream of the U6-siRNA expression cassette. This allows simultaneous expression of both shRNA and selectable marker. dsRed is co-integrated into host cell genome with shRNA which allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox 3.7 Puro
rHIV Pro-viral plasmid used in shRNA construction containing a puromycin selectable marker. U6 promoter is upstream of CMV-Puromycin expression cassette for simultaneous production of shRNA and selectable marker. Puromycin co-integrates into host cells genome which allows for convenient, stable selection.

pLentilox RSV GFP

rHIV Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for GFP marker utilizing a CMV promoter. GFP is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox RSV2dsRed rHIV Transgene expression of this proviral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for dsRed marker utilizing a CMV promoter. dsRed is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and selection through fluorescent visualization.
pLentilox RSV puro

 
rHIV Transgene expression of this pro-viral plasmid is driven by an RSV promoter. Vector also contains separate expression cassette for puro marker utilizing a CMV promoter. Puro is co-integrated into host cell genome and allows for convenient tracking of viral uptake in the cell and puromycin selection.
pLentilox IRES GFP

 
rHIV Proviral plasmid containing a bicistronic element to express both a transgene and GFP marker driven by one CMV promoter through the use of an IRES element. IRES elements tend not to be as strong as typical promoter expression cassettes and therefore could result in a weaker GFP signal.
pLentilox IRES Puro

 
rHIV Proviral plasmid containing a bicistronic element to express both a transgene and a puromycin selectable marker driven by one CMV promoter through the use of an IRES element. IRES elements tend not to be as strong as typical promoter expression cassettes and therefore could result in a weaker puromycin response.

pLentilox EV

rHIV Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains no selectable marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.

pLenti6/V5-DEST

N/A rHIV This Invitrogen expression system utilizes the Gateway Technology of gene transfer. This vector is ideal for multiple expression systems. Contains blasticidin resistance gene for stable selection. This plasmid is fully compatible with Vector Core system for custom lentivirus production.
pLenti6/V5-GW/LacZ N/A rHIV This Invitrogen expression system utilizes the Gateway Technology of gene transfer. This vector allows simple subcloning of transgene. Blasticidin resistance gene for stable selection. This plasmid is fully compatible with Vector Core system for custom lentivirus production.
pLentilox EV-GFP
N/A
rHIV Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains the GFP marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.
pLentilox EV-Luc
N/A
rHIV Proviral plasmid in which transgene expression is driven by a CMV promoter. Vector contains the luciferase (firefly) marker. The use of the CMV promoter is ideal for transgene over-expression in a wide range of cell types.
Lenti-FIV-GFP N/A N/A

rFIV

Recombinant Feline Immunodeficiency Virus (FIV) reporter vector expressing the GFP marker. Expression is driven from an RSV promoter.
Lenti-FIV-LacZ N/A N/A

rFIV

Recombinant Feline Immunodeficiency Virus (FIV) reporter vector expressing the β-gal marker. Expression is driven from an RSV promoter.

***Non-University of Michigan customers: please contact Aldevron to purchase stock pUMVC expression plasmids.

Note to all Vector Core customers: the pNGVL plasmids have been renamed as pUMVC. The plasmids are still available to all investigators, but the reference name has changed. If you have any questions please contact the lab supervisor Tom Lanigan at lanigant@umich.edu.

pUMVC Expression Plasmids

Name Map Sequence Description
pUMVC1-CAT
N/A
Mammalian expression vector for the expression of chloramphenicol resistance. CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – chloramphenicol transferase.
pUMVC1-hGM-CSF
N/A
CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – human granulocyte-monocyte colony stimulating factor.
pUMVC1-hpAP
N/A
CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – human placental alkaline phosphatase.
pUMVC1-mGM-CSF
N/A
 CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – mouse granulocyte-monocyte colony stimulating factor.
pUMVC1-nt-beta-gal 
N/A
 CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – nuclear targeted beta-galactosidase.
pUMVC1-tk
N/A
 CMV IE Promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – mammalian expression vector for HSV1 thymidine kinase.
pUMVC3-4070a-en
N/A
Expresses Moloney mouse leukemia virus gPr80 envelope polyprotein precursor. This precursor is proteolytically cleaved into gp70SU and p15TM subunits. Amphotropic 4070A virus envelope.
pUMVC3-gag-pol 
N/A
Plasmid expresses gag-pol genes from Moloney murine leukemia virus for co-tranfection with expression vector and appropriate env expressing plasmid. Retrovirus gag-pol helper plasmid, kanamycin resistant.
pUMVC3-hFL
N/A
 Mammalian expression vector for human Flt3 ligand, full length.
pUMVC3-hFLex
N/A
 Mammalian expression vector for human Flt3 ligand, secreted.
pUMVC3-hIFN alpha 2
N/A
 CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, expresses human interferon alpha 2.
pUMVC3-hIL10
N/A
 Mammalian expression vector for human interleukin 10.
pUMVC3-hIL12
N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, internal ribosome entry site, p35, p40 – human interleukin-12.
pUMVC3-hIL15
N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance – mammalian expression vector for human interleukin-15.
pUMVC3-hIL2
N/A
 CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – mammalian expression vector for human interleukin-2
pUMVC3-hIL2/IL15 
N/A
Human interleukin 2 signal peptide fused to human interleukin15 mature peptide. CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance, human interleukin-2/interleukin-15 fusion.
pUMVC3-hIL4
N/A
 A mammalian expression vector for human interleukin 4.
pUMVC3-hIL7 
N/A
 Mammalian expression vector for human interleukin 7.
pUMVC3-mFL 
N/A
Mammalian expression vector for mouse FLT3 ligand.
pUMVC3-mFLex
N/A
Mammalian expression vector expressing a secreted form of mouse FLT3 ligand.
pUMVC3-mIL10
N/A
 Mammalian expression vector for mouse interleukin 10, CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal.
pUMVC3-mIL12
N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, internal ribosome entry site, p35, p40 – mammalian expression vector for mouse interleukin 12.
pUMVC3-mIL2
N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – mammalian expression vector for mouse interleukin 2.
pUMVC3-mIL4
N/A
Mammalian expression vector for mouse interleukin 4.
pUMVC3-mIL7
N/A
Mammalian expression vector for mouse interleukin 7.
pUMVC3-shIL15R
N/A
Expression vector for the extracellular domain of human interleukin 15 receptor alpha -soluble human interleukin 15 receptor alpha.

 

Expression Vectors

Name Map Sequence Description

pUMVC1

N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – PstI, SalI, EcoRV, BglII polylinker, kanamycin resistance.
pUMVC2
N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal – Expression plasmid, CMV promoter, polylinker deleted, rabbit beta-globin poly A, kanamycin resistance.
pUMVC3
N/A
 Mammalian expression plasmid CMV IE promoter/ enhancer/trimmed Intron A, rabbit beta-globin polyadenylation signal –  kanamycin resistance.
pUMVC4a
N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance, ISS from ampicillin resistance gene (+) orientation – immunostimulatory sequence.
pUMVC4b 
N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance, ISS from ampicillin resistance gene (-) orientation – immunostimulatory sequence.
pUMVC6
N/A
CMV IE promoter/enhancer/ trimmed Intron A, rabbit beta-globin polyadenylation signal, kanamycin resistance, modified interleukin-2 secretion signal sequence – secretion signal peptide, immunostimulatory sequence.
pUMVC7
N/A
Can be used to retarget intracellular peptides to a secretory pathway by fusion to the tissue plasminogen activator signal peptide. – pUMVC7, CMV promoter, tPA signal peptide.
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