We are excited to announce the incorporation of Illumina’s NovaSeq 6000 into the DNA Sequencing Core’s stable of sequencing instruments. As a testament to the cutting-edge nature of U-M research, ours is one of the first academic core facilities to house this exciting and powerful technology. A wide variety of libraries – including whole genome, whole exome, mRNA, ribo-depleted RNA, ATAC, WGBS and more – are now routinely sequenced on the NovaSeq. We have also transitioned our 10X Chromium Single Cell workflow to NovaSeq.
We are revising our procedures to ensure that you maximally benefit from the efficiency, data quality, and cost savings of the NovaSeq. Beginning Monday, April 1, 2019, you will encounter a new sample submission process. You will select the number of cycles and total reads you require for your submission (no more lanes!). Libraries prepped in the core will use unique dual indices to help prevent index collisions and hopping and to help you to share flow cells. We strongly encourage users making their own libraries to also incorporate a dual indexing strategy. When protocols are not amenable to dual indices, please reach out to discuss best practices.
As always, we are happy to help you plan your experiments to make best use of the enhanced capabilities of the NovaSeq – we think you’re going to like it. Please contact firstname.lastname@example.org with any questions. Also, please check out our NGS FAQs.
Thank you for choosing the U-M DNA Sequencing Core for your genomics needs.
Olivia Koues, Managing Director
Tom Wilson, Faculty Director
U-M DNA Sequencing Core
The NovaSeq 6000 combines 2 color SBS chemistry with the nanowell patterned flow cell. There are four flow cells options, SP, S1, S2, and the S4. There are two different ways to load the Flow cell, individual lane loading (XP) so as to run various experiments on the same flow cell or whole flow cell loading for one experiment across all lanes for greater coverage. We have compiled Illumina specifications for the NovaSeq in the tables below. To see a list of common and compatible sequencing methods for the NovaSeq 6000 visit Illumina’s website or reach out to the DNA Sequencing core for help with your project.
|Flow cell Type||Paired-End Reads||Read length||Output||Quality Scores||Run Time|
||100 cycles||65-80 GB||≥85%||~13 hr|
|300 cycles||200-250 Gb||≥75%||~25 hr|
|500 cycles||325-400 Gb||≥75%||~38 hr|
||100 cycles||134-167 Gb||≥85%||~13 hr|
|200 cycles||266-333 Gb||≥80%||~19hr|
|300 cycles||400-500 Gb||≥75%||~25hr|
|S2||3.6 billion||100 cycles||333-417 Gb||≥85%||~16hr|
|200 cycles||667-833 Gb||≥80%||~25hr|
|300 cycles||1000-1250 Gb||≥75%||~36hr|
|S4||10 billion||200 cycles||1600-2000Gb||≥80%||~36hr|
|300 cycles||2400-3000 Gb||≥75%||~45hr|
For More Information
You can read more about the NovaSeq 6000 specs at the Illumina web site.
Updated 3/19/19 – OKoues