DNA/RNA QC : Qubit Fluorometer

The Qubit 2.0 Fluorometer is capable of measuring the concentration of nucleic acids using Life Technologies Qubit® DNA and RNA quantitation assays. In contrast to the nanodrop, The Qubit® Quantitation Platform, uses fluorescent dyes to measure the concentration of the specific molecules of interest (dsDNA, total nucleic acid, RNA).

PlatformQubit 2.0 Fluorometer
CategoryDNA and RNA QC
Primary ContactChristopher Krebs, Manager, UM Advanced Genomics Core
734-763-8891 ckrebs@umich.edu
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical OutputYou will get back the sample's concentration in a Microsoft Excel spreadsheet.
How to RequestLogin through https://client-seqcore.brcf.med.umich.edu and then choose “Sample QC” to submit samples
Sample RequirementsWe request a minimum of 5ul of each sample for analysis. The samples for “QC only” should be in a separate tube from the portion of sample intended for other services within the core. This assists us in minimizing freeze thaw cycles.
Typical Turnaround24 to 48 hrs
Critical InformationAssay Ranges:
Qubit RNA Assay: 0.25 - 100 ng/ul
Qubit High Sensitivity dsDNA Assay: 0.01 - 100 ng/ul
Qubit Broad Range dsDNA Assay: 0.1 – 1000 ng/ul
Qubit ssDNA Assay: 0.05 – 200 ng/ul
More InformationThe Qubit® 2.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. Qubit® fluorometric quantitation provides the most specific and sensitive DNA and RNA quantitation available, even at low concentrations

Link to additional information about the Qubit : Qubit® 2.0 Fluorometer