Introduction to the UM DNA Sequencing Core
General Overview:The University of Michigan DNA Sequencing Core was created to provide UM, and the broader research community, access to a wide range of technologically-advanced nucleic acid analytical methods. We are located in UM’s state-of-the-art North Campus Research Complex and our staff of talented specialists takes great pride in the variety of services we offer and the high level of quality in the data we generate.
Services: This Core provides analyses that would be difficult to carry out in individual labs, either due to the expense of the required equipment or due to the level of expertise required to perform the analysis. Specific examples include:
- Sanger sequencing
- Next Generation Sequencing
- Illumina NovaSeq
- Illumina HiSeq
- Illumina NextSeq
- Illumina MiSeq
- Microarray analysis
- Illumina microarrays (SNP typing, gene expression)
- Affymetrix microarrays (SNP typing, gene expression)
- Sequenom SNP typing
- Qiagen Pyromark epigenetic analysis
- DNA isolation from blood or saliva
- DNA and RNA quality testing via Agilent Bioanalyzer, TapeStation, NanoDrop and Qubit
- Fragment sizing (e.g. CODIS genotyping, AFLP)
- qPCR instrument access
Support: The Core also offers a variety of support services to help our clients get and keep grant funding, design and troubleshoot experiments, and interpret the outcomes.
- Consultation: At no charge, we will work with our clients, helping them with experimental design. We can often help you choose a path among seemingly-similar analytical methods. In certain cases, we can provide data interpretation as well. See the “Services” and “Platforms” options for specific contact information.
- Sample quality control: we have considerable resources and equipment for assessing the concentration and quality of your DNA or RNA samples.
- Letters of Support: When you write a grant proposal, ask the Core Director for a Letter of Support. We will help you demonstrate that you have the necessary resources and expertise to carry out a complex experiment involving any of our technologies.
- Site visits: If your funding agency is site-visiting, please allow us to help by demonstrating our commitment to technological excellence with a tour of our facility. Contact the Core Director to arrange for a tour.
Who Can Submit Samples?
Our services are primarily intended to benefit the University of Michigan research community, but we do accept samples from outside the UM. You must set up an account with the Core. For UM clients, please see this FAQ; for outside clients, please see this FAQ.
We do not perform testing for the lay public, i.e. we cannot provide paternity testing, ancestry testing, genetic testing for health-related issues or forensic testing to be used in court cases.