Rapid Paired end 50bp Sequencing


Rapid mode paired end sequencing on the HiSeq 2500 that produces paired 50 bp reads.

PlatformIllumina HiSeq Sequencing
CategoryRapid Paired-end 50bp Sequencing
Primary Contactillumina-manager@umich.edu
Typical CostClick here to view internal pricing list!
Click here to view external pricing list!
Typical Output13 Gb per lane

*These are just estimates of the output, these are not guarantees.
How to RequestSample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’
Sample Requirements30 ul of 10nM Illumina library with an insert size of at least 350 bp.
Run TimeThe run itself takes 16 hours the flow cell needs to be full for us to run it. Each flow cell has two lanes and we need to have a rapid mode HiSeq available. This could take some time to fill and run.
Critical InformationRapid modes come at a premium while they are much faster run times they produce about 80% of the data as compared to a high output run.

It is important that we know if you are working with an unbalanced genome or have modified your library prep in any way. 
Please include this information in the notes section when creating a submission.