Rapid Paired end 50bp Sequencing
Rapid mode paired end sequencing on the HiSeq 2500 that produces paired 50 bp reads.
|Platform||Illumina HiSeq Sequencing|
|Category||Rapid Paired-end 50bp Sequencing|
|Typical Cost||Click here to view internal pricing list!
Click here to view external pricing list!
|Typical Output||13 Gb per lane
*These are just estimates of the output, these are not guarantees.
|How to Request||Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’|
|Sample Requirements||30 ul of 10nM Illumina library with an insert size of at least 350 bp.|
|Run Time||The run itself takes 16 hours the flow cell needs to be full for us to run it. Each flow cell has two lanes and we need to have a rapid mode HiSeq available. This could take some time to fill and run.|
|Critical Information||Rapid modes come at a premium while they are much faster run times they produce about 80% of the data as compared to a high output run.
It is important that we know if you are working with an unbalanced genome or have modified your library prep in any way.
Please include this information in the notes section when creating a submission.