Light microscopes can not easily image where visible light can not penetrate. Tissue clearing protocols make tissue highly transparent to enable deep and large volume tissue imaging. We offer RTF and iDisco protocols on a fee for service basis. Other protocols can also be considered. Please contact us with questions as different protocols are better for different kinds of fluorophores and samples.
This example shows two mouse brains, one with and one without the iDisco clearing protocol.
Expansion microscopy cross links the proteins in the sample to a polymer backbone and then swells the polymer mesh to make the sample larger (usually by 4x in each dimension), thus enabling super resolution imaging on a standard fluorescence microscope. We offer the MAP and proExM protocols on a fee for service basis. Please contact us with questions.