Transgenic Animal Model

Build custom mouse and rat models with world-renowned experts

About the Core

 

The University of Michigan Transgenic Animal Model Core was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of genome editing (CRISPR/Cas9), transgenic and gene targeting research from experimental design to mouse breeding is freely available. The Core’s mission is to provide access to a complex technology so that investigators can focus their resources on research instead of developing tools for research.

The Transgenic Core routinely uses CRISPR/Cas9 to produce gene edited mice and rats. The Transgenic Core guarantees that at least three transgenic founder mice or rats will be produced for each transgene DNA construct. Since 1989 the Core has produced more than 18,000 transgenic mouse founders from more than 1,800 transgenes and more than 800 transgenic rat founders from 63 transgenes. Over 200 mouse strains were produced by gene targeting in ES cells and another 60 strains from ES cells obtained from the International Knockout Mouse Consortium. The Core uses BAC recombineering to prepare genetically modified bacterial artificial chromosomes (BACs). BACs are genetically modified by homologous recombination (BAC recombineering) to prepare DNA mutations, insertions, and deletions. BACs can be used as transgenes to express reporter molecules (GFP), Cre recombinase, and mutant alleles in cell culture and mice. The also prepares gene targeting vectors for the modification of chromosomes in ES cells. The efficiency of transgenic mouse and rat model production meets or exceeds standards in the published literature. Hundreds of research publications have been based on the use of these mouse and rat models.

Process

Consultation

Consultations are provided at no cost. We provide advice, as well as protocols and training, for every step of the process from experimental design to mouse breeding. Please contact us at any time with questions on how to proceed.

Project Request

To submit a service request, please log-in to MiCores.

SUBMISSIONS

Once prepared, submit all of your materials to the Core so that we may initiate your request.

Transgenic Core by the Numbers

Total PIs (FY18)

Grants Serviced (FY18)

Transgenic mouse founders produced since 1989

Events

  • Explore New Technologies at the BRCF Genomics Showcase

    Join the BRCF for a Genomics Showcase on Thursday, June 13 to explore the rapidly evolving technologies, applications, and platforms available in the genomics space, including single cell analysis, ATAQ-Seq, epigenetics, high-throughput sequencing applications, and more. Thursday, June 13, 2019 …

     
  • Researchpalooza 2019

    Mark your calendars for the return of Researchpalooza! Join the BRCF and the UMMS Office of Research for Researchpalooza during the annual Michigan Medicine Ice Cream Social, with 80+ exhibitors from labs and offices of the Medical School and across …

     

News

  • DNA Sequencing Core Decommissioning the HiSeq4000

    In order to focus on new and emerging technologies, the DNA Sequencing Core will be discontinuing HiSeq4000 services on June 15, 2019. Samples requiring HiSeq4000 sequencing should be submitted as soon as possible to ensure adequate time for processing prior …

       
  • In Focus: Using Single Cell Analysis to Study the Role of Immune Cells in Bone Regeneration

    Dr. Benjamin Levi Single cell analysis–studying genomics at the level of a single cell–has been an important tool for researchers like Benjamin Levi, MD–Assistant Professor in the Department of Plastic Surgery and Director of the Burn/Trauma and Regenerative Medicine Laboratory. …