Transgenic Animal Model

The Transgenic Core collaborates with the BAC Recombineering Core to prepare genetically modified bacterial artificial chromosomes (BACs). The mission of the BAC Core is to provide access to a complex technology to investigators at the so that they can use their resources to conduct research instead of developing tools for research. BACs are genetically modified by homologous recombination (BAC recombineering) to prepare DNA mutations, insertions and deletions. BACs can be used as transgenes to express reporter molecules (GFP), Cre recombinase, and mutant alleles in cell culture and mice. The BAC Core also prepares gene targeting vectors for the modification of chromosomes in ES cells.

 

The Transgenic mouse core guarantees that at least three transgenic founder mice will be produced for each DNA construct. The overall average is ten founders. Since 1989 over 14,000 mouse transgenic founders were produced from more than 1,400 plasmid and BAC transgenes. Nearly 1000 transgenic rat founders have been produced, including BAC transgenic rat strains. Over 200 new mutant mouse models were produced by the electroporation of mouse ES cells with targeting vectors and another 60-odd strains were produced from ES cell clones obtained from the international mouse knock out project (KOMP). Some of these mouse and rat models have been published.