Transgenic Animal Model


The Transgenic Animal Model Core is continuing to operate, with increased cleanliness requirements for equipment, surfaces, supplies, etc. If we reach the point where the university announces an official reduction in operations, this will be reassessed and access to the facilities may be limited or closed. Please note that the University of Michigan will not accept orders for animals during the COVID-19 situation (https://research.umich.edu/covid-19). The Transgenic Core will continue production of new genetically engineered mice and rats after animal ordering resumes.  The CRISPR-Cas9 ES Cell Training Classes scheduled for May and July have not been canceled and we intend to go forward with them. Registered attendees will be notified in advance should this situation change. Please contact Thom Saunders or Elizabeth Hughes for updates regarding specific core access.

Build custom mouse and rat models with world-renowned experts

About the Core

 

The University of Michigan Transgenic Animal Model Core was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of genome editing (CRISPR/Cas9), transgenic and gene targeting research from experimental design to mouse breeding is freely available. The Core’s mission is to provide access to a complex technology so that investigators can focus their resources on research instead of developing tools for research.

The Transgenic Core routinely uses CRISPR/Cas9 to produce gene edited mice and rats. The Transgenic Core guarantees that at least three transgenic founder mice or rats will be produced for each transgene DNA construct. Since 1989 the Core has produced more than 18,000 transgenic mouse founders from more than 1,800 transgenes and more than 800 transgenic rat founders from 63 transgenes. Over 200 mouse strains were produced by gene targeting in ES cells and another 60 strains from ES cells obtained from the International Knockout Mouse Consortium. The Core uses BAC recombineering to prepare genetically modified bacterial artificial chromosomes (BACs). BACs are genetically modified by homologous recombination (BAC recombineering) to prepare DNA mutations, insertions, and deletions. BACs can be used as transgenes to express reporter molecules (GFP), Cre recombinase, and mutant alleles in cell culture and mice. The also prepares gene targeting vectors for the modification of chromosomes in ES cells. The efficiency of transgenic mouse and rat model production meets or exceeds standards in the published literature. Hundreds of research publications have been based on the use of these mouse and rat models.

Process

Consultation

Consultations are provided at no cost. We provide advice, as well as protocols and training, for every step of the process from experimental design to mouse breeding. Please contact us at any time with questions on how to proceed.

Project Request

To submit a service request, please log-in to MiCores.

SUBMISSIONS

Once prepared, submit all of your materials to the Core so that we may initiate your request.

Transgenic Core by the Numbers

Total PIs (FY18)

Grants Serviced (FY18)

Transgenic mouse founders produced since 1989

Events

  • Automating and Extending FIJI With Macros

    An interactive introduction on how to automate image processing and analysis using the FIJI macro language. Attendees are asked to bring a laptop to work along with the examples. Basic experience using FIJI (e.g. IAN01) or exposure to a computer …

     

News

  • Update on Core Access Due to COVID-19

    As you have likely heard, in compliance with the directive from the University of Michigan’s Office of Research, Biomedical Research Core Facilities (BRCF) has completely closed cores as of 5 p.m. Friday, March 20, 2020. For the foreseeable future, the university will …

     
  • Coronavirus (COVID-19) Updates

    March 18, 2020 In compliance with the directive from the University of Michigan’s Office of Research, the Biomedical Research Core Facilities (BRCF) will completely close cores as of 5 p.m. Friday, March 20, 2020. Upon the need to conduct research …