The Transgenic Animal Model Core

was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of genome editing (CRISPR/Cas9), transgenic, and gene targeting research from experimental design to mouse breeding is freely available. The Core’s mission is to provide access to a complex technology so that investigators can focus their resources on research instead of developing tools for research.

Our Process

Consultation

Consultations are provided at no cost. We provide advice, as well as protocols and training, for every step of the process from experimental design to mouse breeding. Please contact us at any time with questions on how to proceed.

Project Request

To submit a service request, please log into MiCores.

Submissions
Once prepared, submit all of your materials to the Core so that we may initiate your request.
What We Do

The Transgenic Core routinely uses CRISPR/Cas9 to produce gene-edited mice and rats. The Transgenic Core guarantees that at least three transgenic founder mice or rats will be produced for every transgene DNA construct. Since 1989 the Core has produced more than 20,000 transgenic mouse founders from more than 2,000 transgenes and more than 1,000 transgenic rat founders. Since 2013 the Core has produced over 3,000 transgenic mouse founders from over 300 CRISPR/Cas9 projects, 16 CRISPR/Cas9 mediated rat models have been completed. CRISPR/Cas9 technology was used for gene knockouts, codon changes, epitope tags, floxed genes, reporter knockins (EGFP, Cre recombinase, etc.), ROSA26 knockins, and specialized projects (regulatory sequence knockouts, ES cell engineering, etc.). Over 200 mouse strains have been produced for genes targeted in ES cells in the Core and another 60 strains from ES cells obtained from the International Knockout Mouse Consortium. In addition, the Transgenic Core offers a full suite of cryopreservation and assisted reproduction (IVF) services.

The efficiency of transgenic mouse and rat model production meets or exceeds standards in the published literature.

General Information

734-647-2910

Our Locations:

Medical Sciences Research Building (MSRB) II

News

  • Our Mission is Your Research

    Over the last two years, the BRCF Communication + Culture and Administrative teams have taken a closer look at what drives our work. Our teams held collaborative working sessions, conducted a department-wide survey to source feedback, and hosted small, internal …

     
  • AGC Data Delivery Changes in the Time of UM-RCP

    With the recent announcement of the University of Michigan Research Computing Package (UMRCP), the BRCF Advanced Genomics Core (AGC) has been working with Information and Technology Services (ITS) to update our data delivery strategy. The Michigan Medicine version of the …

     

Events

  • Bioinformatics Core Virtual 3-Day Workshop: RNA-Seq Demystified

    This is a 3 x ½ day virtual, hands-on workshop targeted toward researchers who would like to be able to run RNA-Seq analysis on their own. Workshop Schedule: 11/15-11/17, 9:00 AM-1:00 PM ET: Hands-On RNA-Seq Analysis About the Workshop: This …

     
  • University of Michigan Multiome Webinar Series – Session 2

    Deep insights into tumor biology, developmental biology, and other biological processes and disease states require a comprehensive view of gene expression patterns and their corresponding epigenetic regulation at single-cell resolution. Leverage two modalities at once in single cells with Chromium …