The Transgenic Animal Model Core

was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of genome editing (CRISPR/Cas9), transgenic, and gene targeting research from experimental design to mouse breeding is freely available. The Core’s mission is to provide access to a complex technology so that investigators can focus their resources on research instead of developing tools for research.

Our Process

Consultation

Consultations are provided at no cost. We provide advice, as well as protocols and training, for every step of the process from experimental design to mouse breeding. Please contact us at any time with questions on how to proceed.

Project Request

To submit a service request, please log into MiCores.

Submissions
Once prepared, submit all of your materials to the Core so that we may initiate your request.
What We Do

The Transgenic Core routinely uses CRISPR/Cas9 to produce gene-edited mice and rats. The Transgenic Core guarantees that at least three transgenic founder mice or rats will be produced for every transgene DNA construct. Since 1989 the Core has produced more than 20,000 transgenic mouse founders from more than 2,000 transgenes and more than 1,000 transgenic rat founders. Since 2013 the Core has produced over 3,000 transgenic mouse founders from over 300 CRISPR/Cas9 projects, 16 CRISPR/Cas9 mediated rat models have been completed. CRISPR/Cas9 technology was used for gene knockouts, codon changes, epitope tags, floxed genes, reporter knockins (EGFP, Cre recombinase, etc.), ROSA26 knockins, and specialized projects (regulatory sequence knockouts, ES cell engineering, etc.). Over 200 mouse strains have been produced for genes targeted in ES cells in the Core and another 60 strains from ES cells obtained from the International Knockout Mouse Consortium. In addition, the Transgenic Core offers a full suite of cryopreservation and assisted reproduction (IVF) services.

The efficiency of transgenic mouse and rat model production meets or exceeds standards in the published literature.

General Information

734-647-2910

Our Locations:

Medical Sciences Research Building (MSRB) II

News

  • Dr. Dolinoy Featured in Society of Toxicology’s new Adverse Reactions Podcast

    Epigenetics expert Dana C. Dolinoy of the University of Michigan School of Public Health and Faculty Director for the BRCF Epigenomics Core illuminates how our nascent understanding of the epigenome is leading to breakthroughs in understanding the causes—and potential treatment—of some …

       
  • Standing Together Against Racism

    Michigan Medicine leadership recently issued the statement that “we unequivocally recognize racism as a public health issue and we stand together against bias and inequality.” Here at the Medical School Office of Research, we wholeheartedly endorse this message and the …

     

Events

  • Introducing StarBright Dyes, Exceptionally Bright & Stable Dyes

    Presented by Bio-Rad and the BRCF Flow Cytometry Core Please join us for this informative data-driven seminar highlighting the utility and capabilities of our new StarBright franchise of flow cytometry dyes. We will show how these dyes can help improve …

     
  • Introduction to Visium Data Analysis

    Presented by 10x Genomics and the BRCF Advanced Genomics Core Build a more comprehensive multidimensional understanding of complex biological systems with Visium Spatial solutions from 10x Genomics. Analyzing datasets that measure different molecular readouts—such as RNA, protein, and morphology—in a …