Transgenic Animal Model
The Transgenic Animal Model Core
was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of genome editing (CRISPR/Cas9), transgenic, and gene targeting research from experimental design to mouse breeding is freely available. The Core’s mission is to provide access to a complex technology so that investigators can focus their resources on research instead of developing tools for research.
Consultations are provided at no cost. We provide advice, as well as protocols and training, for every step of the process from experimental design to mouse breeding. Please contact us at any time with questions on how to proceed.
What We Do
The Transgenic Core routinely uses CRISPR/Cas9 to produce gene-edited mice and rats. The Transgenic Core guarantees that at least three transgenic founder mice or rats will be produced for every transgene DNA construct. Since 1989 the Core has produced more than 20,000 transgenic mouse founders from more than 2,000 transgenes and more than 1,000 transgenic rat founders. Since 2013 the Core has produced over 3,000 transgenic mouse founders from over 300 CRISPR/Cas9 projects, 16 CRISPR/Cas9 mediated rat models have been completed. CRISPR/Cas9 technology was used for gene knockouts, codon changes, epitope tags, floxed genes, reporter knockins (EGFP, Cre recombinase, etc.), ROSA26 knockins, and specialized projects (regulatory sequence knockouts, ES cell engineering, etc.). Over 200 mouse strains have been produced for genes targeted in ES cells in the Core and another 60 strains from ES cells obtained from the International Knockout Mouse Consortium. In addition, the Transgenic Core offers a full suite of cryopreservation and assisted reproduction (IVF) services.
The efficiency of transgenic mouse and rat model production meets or exceeds standards in the published literature.
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