Transgenic Animal Model

Build custom mouse and rat models with world-renown experts

 

About the Core

 

The University of Michigan Transgenic Animal Model Core was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of transgenic and gene targeting research from experimental design to mouse breeding is freely available.
The Transgenic Core collaborates with the BAC Recombineering Core to prepare genetically modified bacterial artificial chromosomes (BACs). The mission of the BAC Core is to provide access to a complex technology to investigators at the so that they can use their resources to conduct research instead of developing tools for research. BACs are genetically modified by homologous recombination (BAC recombineering) to prepare DNA mutations, insertions and deletions. BACs can be used as transgenes to express reporter molecules (GFP), Cre recombinase, and mutant alleles in cell culture and mice. The BAC Core also prepares gene targeting vectors for the modification of chromosomes in ES cells.

 

The Transgenic mouse core guarantees that at least three transgenic founder mice will be produced for each DNA construct. The overall average is ten founders. Since 1989 over 14,000 mouse transgenic founders were produced from more than 1,400 plasmid and BAC transgenes. Nearly 1000 transgenic rat founders have been produced, including BAC transgenic rat strains. Over 200 new mutant mouse models were produced by the electroporation of mouse ES cells with targeting vectors and another 60-odd strains were produced from ES cell clones obtained from the international mouse knock out project (KOMP). Some of these mouse and rat models have been published.

Process

Consultation

Consultations are provided at no cost. We provide advice, as well as protocols and training, for every step of the process from experimental design to mouse breeding. Please contact us at any time with questions on how to proceed.

Project Request

To submit a service request, please log-in to MiCores.

SUBMISSIONS

Once prepared, submit all of your materials to the Core so that we may initiate your request.

Transgenic Core by the Numbers

Total PIs (FY17)

Grants Serviced (FY17)

Transgenic mouse founders produced since 1989

Events

  • Tools & Technology Seminar Series – Andrei Lomize

    The DCMB Tools and Technology Seminar Series is presented in room 2036, Palmer Commons each Thursday, at 12 noon EST. Each seminar highlights a tool or technology, that is under development or in current use, that is of interest to …

     
  • Tools & Technology Seminar Series – Matt Cybulsky

    The DCMB Tools and Technology Seminar Series is presented in room 2036, Palmer Commons each Thursday, at 12 noon EST. Each seminar highlights a tool or technology, that is under development or in current use, that is of interest to …

     

News

  • Microscopy & Image Analysis Laboratory Phasing Out Histology Services

    In order to better focus on providing access to state-of-the-art microscopy imaging and analysis, the Microscopy & Image Analysis Laboratory (MIL) is phasing out all light microscopy histology services, including cryosectioning, vibratome sectioning, and paraffin processing/embedding/sectioning. Importantly, the MIL will continue to …

     
  • Medical School Office of Research Seeks a Faculty Director for the BRCF DNA Sequencing Core

    The current and founding Director of the DNA Sequencing Core, Bob Lyons, Ph.D., will be retiring in September. To that end, the Medical School Office of Research is accepting nominations for a Faculty Director for the Biomedical Research Core Facilities …