Transgenic Animal Model

Build custom mouse and rat models with world-renown experts

 

About the Core

 

The University of Michigan Transgenic Animal Model Core was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of transgenic and gene targeting research from experimental design to mouse breeding is freely available.
The Transgenic Core collaborates with the BAC Recombineering Core to prepare genetically modified bacterial artificial chromosomes (BACs). The mission of the BAC Core is to provide access to a complex technology to investigators at the so that they can use their resources to conduct research instead of developing tools for research. BACs are genetically modified by homologous recombination (BAC recombineering) to prepare DNA mutations, insertions and deletions. BACs can be used as transgenes to express reporter molecules (GFP), Cre recombinase, and mutant alleles in cell culture and mice. The BAC Core also prepares gene targeting vectors for the modification of chromosomes in ES cells.

 

The Transgenic mouse core guarantees that at least three transgenic founder mice will be produced for each DNA construct. The overall average is ten founders. Since 1989 over 14,000 mouse transgenic founders were produced from more than 1,400 plasmid and BAC transgenes. Nearly 1000 transgenic rat founders have been produced, including BAC transgenic rat strains. Over 200 new mutant mouse models were produced by the electroporation of mouse ES cells with targeting vectors and another 60-odd strains were produced from ES cell clones obtained from the international mouse knock out project (KOMP). Some of these mouse and rat models have been published.

Process

Consultation

Consultations are provided at no cost. We provide advice, as well as protocols and training, for every step of the process from experimental design to mouse breeding. Please contact us at any time with questions on how to proceed.

Project Request

To submit a service request, please log-in to MiCores.

SUBMISSIONS

Once prepared, submit all of your materials to the Core so that we may initiate your request.

Transgenic Core by the Numbers

Total PIs (FY17)

Grants Serviced (FY17)

Transgenic mouse founders produced since 1989

Events

  • Flow Cytometry Core Instrumentation Training Lecture 09-28-18

    This event applies specifically to the Flow Cytometry Core. This lecture is one of two steps for those interested in Flow Core instrumentation training. No training is required to bring samples to the facility to be run by the Flow …

         
  • Flow Cytometry Core Instrumentation Training Lecture 09-11-18

    This event applies specifically to the Flow Cytometry Core. This lecture is one of two steps for those interested in Flow Core instrumentation training. No training is required to bring samples to the facility to be run by the Flow …

         

News

  • In Focus: Paclitaxel-Induced Peripheral Neuropathy and the Role of Complement

    With the help of the Biomedical Research Core Facilities Transgenic Animal Model Core (TAMC), Feng Lin, Ph.D. (Cleveland Clinic Lerner Research Institute) and colleagues may have elucidated the role of complement in the development of chemotherapy-induced peripheral neuropathy (CIPN). CIPN …

       
  • New Services from the Proteomics Core

    The Proteomics & Peptide Synthesis Core is excited introduce two new services now available: Pathway Analysis: The results from protein profiling and expression profiling (e.g. label-free quantitation by spectral counting) can be directly analyzed to elucidate the impact of the detected biomarker …