Transgenic Animal Model

Build custom mouse and rat models with world-renowned experts

 

About the Core

 

The University of Michigan Transgenic Animal Model Core was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of genome editing (CRISPR/Cas9), transgenic and gene targeting research from experimental design to mouse breeding is freely available. The Core’s mission is to provide access to a complex technology so that investigators can focus their resources on research instead of developing tools for research.

The Transgenic Core routinely uses CRISPR/Cas9 to produce gene edited mice and rats. The Transgenic Core guarantees that at least three transgenic founder mice or rats will be produced for each transgene DNA construct. Since 1989 the Core has produced more than 18,000 transgenic mouse founders from more than 1,800 transgenes and more than 800 transgenic rat founders from 63 transgenes. Over 200 mouse strains were produced by gene targeting in ES cells and another 60 strains from ES cells obtained from the International Knockout Mouse Consortium. The Core uses BAC recombineering to prepare genetically modified bacterial artificial chromosomes (BACs). BACs are genetically modified by homologous recombination (BAC recombineering) to prepare DNA mutations, insertions, and deletions. BACs can be used as transgenes to express reporter molecules (GFP), Cre recombinase, and mutant alleles in cell culture and mice. The also prepares gene targeting vectors for the modification of chromosomes in ES cells. The efficiency of transgenic mouse and rat model production meets or exceeds standards in the published literature. Hundreds of research publications have been based on the use of these mouse and rat models.

Process

Consultation

Consultations are provided at no cost. We provide advice, as well as protocols and training, for every step of the process from experimental design to mouse breeding. Please contact us at any time with questions on how to proceed.

Project Request

To submit a service request, please log-in to MiCores.

SUBMISSIONS

Once prepared, submit all of your materials to the Core so that we may initiate your request.

Transgenic Core by the Numbers

Total PIs (FY17)

Grants Serviced (FY17)

Transgenic mouse founders produced since 1989

Events

  • 2018 Biomedical Research Core Facilities Holiday Hours

    As in past years, many staff in the Biomedical Research Core Facilities will be using University Season Days between December 25 and January 1. The schedule for the individual Cores during the holidays is given below. Bioinformatics Core (B22 NCRC): Services …

     

News

  • The DNA Sequencing Core Welcomes New Leadership

    A Message from Steve Kunkel, Ph.D., Interim Chief Scientific Director, and BRCF Leadership  We are pleased to announce that Thomas Wilson MD, Ph.D., Professor of Pathology and Human Genetics, and Olivia Koues, Ph.D., have joined the University of Michigan Medical …

     
  • In Focus: U-M Kidney Research Collaboration Works to Discover New Ways to Treat Kidney Disease

    Featuring Dr. Matthias Kretzler; Warner-Lambert/Parke-Davis Professor of Medicine, Professor of Internal Medicine and Research Professor, Computation Medicine and Bioinformatics Chronic kidney disease (CKD), the progressive decline in kidney function, affects nearly 30 million Americans, with millions of others at increased risk …