Transgenic Rats Are Available from the University of Michigan Transgenic Core
The Transgenic Facility routinely produces transgenic rats for biomedical research. We have developed considerable expertise in this area (Filipiak and Saunders, 2006). The general outline of producing transgenic rats and transgenic mice is the same.
The efficiency of transgenic rat production is equivalent to the production of transgenic mice in inbred mouse strains such as C57BL/6J.
We have generate transgenic rats in the following genetic backgrounds:
Fischer 344 Rats – F344/NHsd
Long Evans Rats – Crl:LE
Sprague Dawley Rats – Crl:CD (SD)
Wistar Rats – Crl:Wi
We have successfully generated:
CRISPR/Cas9 gene knockout and gene knockin (Cre, Flpo, etc.) rat models
Plasmid transgene transgenic rats
Bacterial artificial chromosome transgenic rats
Knockout rats by the pronuclear microinjection of zinc finger nuclease mRNA.
If you are interested in obtaining transgenic rats from the Transgenic Facility, please contact Thom Saunders for more information.