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Providing stable integration in dividing cells, used for the production of permanent cell lines and long-term modifications for in vivo and ex vivo applications.
Standard turn-around time is 2 weeks unless otherwise noted.
| Service | Yield/Amount | Internal Price* | External Price |
| Concentrated Retroviral Production (small scale, VSVG) | 100 mls of retroviral supernatant pseudotyped with VSVG, and concentrated to 10 mls of 10X (or as requested) in DMEM (or as requested); 80 μg of proviral plasmid required. | $678.00 | $1,017.00 |
| UNCONCENTRATED Retroviral Small Scale Prep (100 mls of 1X virus) | For use with MMLV/MSCV plasmids pseudotyped with ecotropic and amphotropic envelopes which are too fragile to concentrate without great loss. Requires 100 μg of proviral DNA. | $564.00 | $846.00 |
| Lentiviral/Retroviral Titer with Fluorescence 200 μl of virus is required. | 1-2 weeks | $369.00 | $553.50 |
| Lentiviral/Retroviral Titer with Antibiotic Selection 200 μl of virus is required. | 2-3 weeks | $417.00 | $625.50 |
| Lentiviral/Retroviral Transduction | Transduction of lentiviral/retroviral constructs onto cells provided by the customer, or on A549 lung carcinoma cells provided by the Vector Core. | $310.00 | $465.00 |
Retrovirus - Ready-Made Stock
ONLY Available as a Custom Viral Prep, and only to UM investigators.
| MoMLV-GFP | Reporter virus expressing a GFP marker for infection optimization. Expression is driven from a CMV promoter. |
Viewable files of maps and sequences for each type.
| Name | Description |
| pRET10 | Proviral plasmid used in the construction of recombinant MoMLV. This plasmid contains a bicistronic element to express both a transgene and GFP marker driven by one CMV promoter through the use of an IRES element. The CMV promoter is followed by an intron region flanked with donor and acceptor splicing sites before the multi-splicing site. |
| pRET6 | Proviral plasmid used in the construction of recombinant MoMLV. This plasmid contains a bicistronic element to express both a transgene and puromycin selectable marker driven by one CMV promoter through the use of an IRES element. The CMV promoter is followed by an intron region flanked with donor and acceptor splicing sites before the multi-splicing site. |
Room C552
Medical Science Research Building (MSRB) II
1150 West Medical Center Drive
Ann Arbor, MI 48109
1150 West Medical Center Drive
Ann Arbor, MI 48109
Phone:: 734-615-1332
Email: [email protected]
The Vector Core is one of the Biomedical Research Core Facilities, and a part of the Medical School Office of Research, where our mission is to foster an environment of innovation and efficiency that serves the Michigan Medicine research community and supports biomedical science from insight to impact.
