Retrovirus
Retroviral Services
| Service | Yield/Amount | Internal Price** | External Price |
| Concentrated Retroviral Production (small scale, VSVG) | 100 mls of retroviral supernatant pseudotyped with VSVG, and concentrated to 10 mls of 10X (or as requested) in DMEM (or as requested); 80 μg of proviral plasmid required. | $678.00 | $1,017.00 |
| UNCONCENTRATED Retroviral Small Scale Prep (100 mls of 1X virus) | For use with MMLV/MSCV plasmids pseudotyped with ecotropic and amphotropic envelopes which are too fragile to concentrate without great loss. Requires 100 μg of proviral DNA. | $564.00 | $846.00 |
| Lentiviral/Retroviral Titer with Fluorescence 200 μl of virus is required. | 1-2 weeks | $369.00 | $553.50 |
| Lentiviral/Retroviral Titer with Antibiotic Selection 200 μl of virus is required. | 2-3 weeks | $417.00 | $625.50 |
| Lentiviral/Retroviral Transduction | Transduction of lentiviral/retroviral constructs onto cells provided by the customer, or on A549 lung carcinoma cells provided by the Vector Core. | $310.00 | $465.00 |
* Standard turn-around time is 2 weeks for retroviral services unless otherwise noted.
** Discounts may be available for members of Centers that support the Vector Core (Cancer – 30%; GI Peptide – 50%).
Retrovirus (Ready-Made Stock)*
| Name | Map | Sequence | Description |
| MoMLV-GFP | N/A | N/A | Reporter virus expressing a GFP marker for infection optimization. Expression is driven from a CMV promoter. |
* ONLY Available as a Custom Viral Prep, and only to UM investigators.
Retroviral Plasmids
| Name | Map | Sequence | Description |
|
pRET10 |
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Proviral plasmid used in the construction of recombinant MoMLV. This plasmid contains a bicistronic element to express both a transgene and GFP marker driven by one CMV promoter through the use of an IRES element. The CMV promoter is followed by an intron region flanked with donor and acceptor splicing sites before the multi-splicing site. |
|
pRET6 |
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|
Proviral plasmid used in the construction of recombinant MoMLV. This plasmid contains a bicistronic element to express both a transgene and puromycin selectable marker driven by one CMV promoter through the use of an IRES element. The CMV promoter is followed by an intron region flanked with donor and acceptor splicing sites before the multi-splicing site. |