The University of Michigan Transgenic Animal Model Core will offer the CRISPR/Cas9 and Mouse ES Cell Training Class from November 4 through November 15. In this two-week long hands-on session trainees will design, build, and validate CRISPR/Cas9 reagents in mouse ES cells. Trainees will learn how to work with mouse embryonic stem cells in culture, to clone CRISPR/Cas9 reagents in plasmids, and to purify and analyze DNA from cultured cells.

Trainees will learn fundamental genetic principles for genetic engineering including:

  1. planning CRISPR/Cas9 genome editing workflows,
  2. fundamental principles of gene targeting design, and
  3. how to apply Cre recombinase technology to conditional genes (“floxed genes”).
In addition, trainees will be introduced to the power of genetically engineered mice to model human disease and learn how to access resources from the International Knockout Mouse Consortium project to knockout every protein coding gene in the mouse. After attending trainees will be able to design experiments to create genetic modifications and produce them in mouse ES cells and in mice with CRISPR/Cas9 reagents.

CRISPR/Cas9 and Mouse ES Cell Training Class
November 4 – November 15
Mouse ES Cell Laboratory, 2578 MSRB II
Course Instructors: Elizabeth Hughes and Thom Saunders

A sample syllabus can be downloaded here.

To reserve a place in this two-week class, please send an email to tsaunder@umich.edu. Places are filled on a first-come first-serve basis. There is a $1900 fee to defray costs.

 

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