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An Introduction to Illumina Sequencing Methods That Require Home-Brew Library Prep Methods, Including ChIP-Seq, ATAC-Seq, HIC, and CRISPR Screening and Critical Factors to Ensure Success
February 25, 2021 @ 12:00 pm - 1:15 pm
Illumina and the University of Michigan Advanced Genomics Core would like to invite you to join us for a workshop series. We will focus on building a foundational knowledge of Illumina Sequencing and Library Prep to ensure your success in many home-brew applications.
In this webinar, we will discuss popular applications that utilize home-brew library preps with Illumina Sequencing as a downstream readout. This will include an overview of epigenetic methods designed to look at regions of open chromatin (ATAC-Seq), binding sites of DNA-associated proteins (ChIP-Seq/Cut and Run), and detection of long-range DNA interactions (HiC). Additionally, we will look at how Illumina Sequencing can be used as an effective strategy in genome-wide CRISPR screens for quantification of sgRNAs as well as methods that allow the detection of transcriptional changes observed in the enriched or depletion knockout populations.