The BRCF Microscopy Core is installing a Zeiss Lattice Light Sheet Microscope on September 14th, which was funded by the Biosciences Initiative.
Lattice light sheet uses a thin sheet of light to rapidly and gently collect high-resolution fluorescence images with exquisite sub-cellular detail. This system is designed for high-resolution, high speed, imaging of cell cultures and the superficial layers of small embryos with virtually no photobleaching or phototoxicity. This system’s speed is also useful for collecting 3D images of thin (<100 um), clear, tissue slabs. Our system will work with most coverslip-bottomed dishes. It is not designed for imaging very large volumes at cellular resolution. It is not a super-resolution technique and it is not equipped with adaptive optics.
Here are a few publications outlining fruitful applications of lattice light sheet microscopy:
- A broad comparison of light sheet technologies: https://www.ncbi.nlm.nih.gov/pubmed/31299171
- Endocytosis dynamics in organoids: https://www.ncbi.nlm.nih.gov/pubmed/30188768
- With expansion microscopy for brain circuitry: https://www.ncbi.nlm.nih.gov/pubmed/30655415
- Single molecule imaging in fly embryos: https://www.ncbi.nlm.nih.gov/pubmed/29956254
- Actin-dynamics during neutrophil migration: https://www.ncbi.nlm.nih.gov/pubmed/28948912
- Organelle interactions: https://www.ncbi.nlm.nih.gov/pubmed/28538724
- Dynamics of T-cell activation: https://www.ncbi.nlm.nih.gov/pubmed/28495700
- PAINT-based super resolution: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889433/
Please plan for how your lab can benefit from the use of this new lattice light sheet microscope. Our long term goal is to train users to use this system on their own. However, due to COVID and for the foreseeable future, please plan to bring us your samples and we will do the imaging while you watch via Zoom. There is a cell culture room next to the microscope that can house living cells or embryos for up to several days.
The system will be completely free until 2021! Please contact me (firstname.lastname@example.org) to further discuss how this new technology can be applied to your research. The University of Michigan’s Microscopy Core is now one of only four sites in the United States to have a Zeiss lattice light sheet so please plan to use this system early and often!